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Mouse Bub1b ELISA Kit

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  • Alternative name

    Mouse MAD3/BUB1-related protein kinase ELISA Kit;Mouse BUBR1 ELISA Kit;Mouse mitotic checkpoint kinase MAD3L ELISA Kit;Mouse Mad3l ELISA Kit;Mouse AU045529 ELISA Kit;Mouse BUB1B, mitotic checkpoint serine/threonine kinase ELISA Kit;Mouse mitotic checkpoint serine/threonine-protein kinase BUB1 beta ELISA Kit;Mouse budding uninhibited by benzimidazoles 1 beta ELISA Kit;Mouse budding uninhibited by benzimidazoles 1 homolog, beta ELISA Kit;
  • Catalog
    E046213
  • species
    Mouse
  • GeneBub1b
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Mouse BUB1B. No significant cross-reactivity or interference between Mouse BUB1B and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity9.38 pg/ml
  • Intended UseMouse Bub1b ELISA Kit allows for the in vitro quantitative determination of Bub1b , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for BUB1B has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any BUB1B present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for BUB1B is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of BUB1B bound in the initial step. The color development is stopped and the intensity of the color is measured.
  • Mouse Mitotic checkpoint serine/threonine-protein kinase BUB1 beta Protein information
  • Uniprot ID BUB1B_MOUSE
  • Uniprot AC Q9Z1S0; A2ARS1;
  • UniGene Mm.29133;
  • GeneID 12236
  • KEGG mmu:12236;
  • Mouse Mitotic checkpoint serine/threonine-protein kinase BUB1 beta Protein SEQUENCE

  • SEQUENCE 1052 AA; 118392 MW; 9EF1ECF3735DD1F4 CRC64;

    MAEASEAMCL EGAEWELSKE NIQPLRHGRV MSTLQGALAK QESAGHTALQ

    QQKRAFESEI RFYSGDDPLD VWDRYINWTE QNYPQGGKES NMSALVERAI

    EALQGETRYY NDPRFLSLWI KLGHLCNEPL DMYSYLQSQG IGVSLAQFYI

    SWAEEYEARE NFKKADIIFQ EGIERKAEPL DRLQSQHRQF QSRVSRQAFL

    ALGNEEEEAL EPSEPQRSSL AELKSRGKKM ARAPISRVGG ALKAPGQSRG

    FLNAVPQPVH GNRRITVFDE NADTASRTEL SKPVAQPWMA PPVPRAKENE

    LQPGPWSTDR PAGRRPHDNP ASVTSIPSVL PSFTPYVEES AQQTVMTPCK

    IEPSINHVLS TRKPGREEGD PLQRVQSHQQ GCEEKKEKMM YCKEKIYAGV

    GEFSFEEIRA EVFRKKLKER REAELLTSAK KREEMQKQIE EMERRLKAMQ

    AVQQEGAGGQ QEEKMPTEDP ARLQIASGPQ EMSGVPLSCS ICPLSSNPRE

    ISPAENILQE QPDSKGSSMP FSIFDESLSD KKDKSPATGG PQVLNAQRRP

    LSVLKTTEVG TTNEDVSPDI CDELTELEPL SEDAIITGFR NVTLCPNPED

    TCDFARAARL ASTPFHEILS SKGIAADPEG LLQEEDLDGK AAEAHHTVHH

    QALIIKKLSP IIEESREATH SSGFSRSSSS APSTSSIKGF QLLEKLELTN

    DGAEDAIQSP WCSQYRLQLL KSLLELSAFA EFSVEDRPMP VLEIGKEIEL

    GPEDYVIKQE HLTCDDYRLF WVAPRSSAEL TMIKASSQPI PWDFYINLKL

    KERLNEDYDQ LCSCCQYQDG HVVWYQYINC STLQNLLQHS EFVTHEIIVL

    IIYNLLTIVE KLHRAEIVHG DLSPRSLILR NRIHDPYDYV NKDDHAVRIM

    DFSYSVDLRV QLDAFAYSGF RTAQILEGQK ILANCSSPYH VDLLGIADLA

    HLLLFKEHLH VFWDGLLWKL SQSTSELKDS ELWNKFFVRI LNASDKSTVS

    VLGELAAEMG GAFDATFHSH LNRALWKLGK TISPEALLTQ QDKQPGGSQS

    PA

  • UCSC uc008lse.2; mouse.;



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