Mouse Bmp7 ELISA Kit

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  • Alternative name

    Mouse Bmp-7 ELISA Kit;Mouse osteogenic protein 1 ELISA Kit;Mouse OP-1 ELISA Kit;Mouse OP1 ELISA Kit;Mouse bone morphogenetic protein 7 ELISA Kit;

  • Catalog
    E045928
  • species
    Mouse
  • GeneBmp7
  • Standard CurveMouse Bmp7 ELISA Kit
  • Other Species Human BMP7 ELISA KitCanine BMP7 ELISA Kit
  • SpecificityNatural and recombinant mouse BMP-7
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity Sample For quantitative detection of mouse BMP-7 in bone tissue, cell culture supernates, s
  • Intended UseMouse Bmp7 ELISA Kit allows for the in vitro quantitative determination of Bmp7 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the assay: Mouse BMP-7 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for BMP-7 has been precoated onto 96-well plates. Standards(Expression system for standard: CHO, Immunogen sequence: S292-H430) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for BMP-7 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse BMP-7 amount of sample captured in plate. Background: Bone morphogenetic protein 7 or BMP7 (also known as osteogenic protein-1 or OP-1) is a protein that in humans is encoded by the BMP7 gene.The protein encoded by this gene is a member of the TGF-beta superfamily. Like other members of the bone morphogenetic protein family of proteins, it plays a key role in the transformation of mesenchymal cells into bone and cartilage. It is inhibited by noggin and a similar protein, chordin, which are expressed in the Spemann-Mangold Organizer. BMP7 may be involved in bone homeostasis. It is expressed in the brain, kidneys and bladder.
  • Mouse Bone morphogenetic protein 7 Protein information
  • Uniprot ID BMP7_MOUSE
  • Uniprot AC P23359; Q91XF7;
  • UniGene Mm.392150; Mm.595;
  • GeneID 12162
  • KEGG mmu:12162;
  • Mouse Bone morphogenetic protein 7 Protein SEQUENCE
  • SEQUENCE 430 AA; 49199 MW; 464F36D5D2E54041 CRC64;

    MHVRSLRAAA PHSFVALWAP LFLLRSALAD FSLDNEVHSS FIHRRLRSQE

    RREMQREILS ILGLPHRPRP HLQGKHNSAP MFMLDLYNAM AVEESGPDGQ

    GFSYPYKAVF STQGPPLASL QDSHFLTDAD MVMSFVNLVE HDKEFFHPRY

    HHREFRFDLS KIPEGEAVTA AEFRIYKDYI RERFDNETFQ ITVYQVLQEH

    SGRESDLFLL DSRTIWASEE GWLVFDITAT SNHWVVNPRH NLGLQLSVET

    LDGQSINPKL AGLIGRHGPQ NKQPFMVAFF KATEVHLRSI RSTGGKQRSQ

    NRSKTPKNQE ALRMASVAEN SSSDQRQACK KHELYVSFRD LGWQDWIIAP

    EGYAAYYCEG ECAFPLNSYM NATNHAIVQT LVHFINPDTV PKPCCAPTQL

    NAISVLYFDD SSNVILKKYR NMVVRACGCH

  • UCSC uc008odb.3; mouse.;



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