Human BDNF ELISA Kit

Alternative name
Human BDNF ELISA Kit;Human abrineurin ELISA Kit;Human ANON2 ELISA Kit;Human BULN2 ELISA Kit;Human brain derived neurotrophic factor ELISA Kit;Human brain-derived neurotrophic factor ELISA Kit;Human neurotrophin ELISA Kit;
Catalog
E004589
species
Human
GeneBDNF
SpecificityNatural and recombinant human BDNF
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity< 2pg/ml
Intended UseHuman BDNF ELISA Kit allows for the in vitro quantitative determination of BDNF , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the assay: human BDNF ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for BDNF has been precoated onto 96-well plates. Standards(sf21, H129-R247) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for BDNF is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human BDNF amount of sample captured in plate. Background: Brain-derived neurotrophic factor (BDNF) is a prosurvival factor induced by cortical neurons that is necessary for survival of striatal neurons in the brain. It is a secreted protein with the molecular weight of 27.8kDa, consisting of 247 amino acids. It is known to promote neuronal survival and differentiation. BDNF shares substantial amino acid sequence identity with nerve growth factor (NGF). BDNF and neurotrophin-3 (NT-3) are two recently cloned neurotrophic factors that are homologous to NGF. mRNA products of the BDNF and NT-3 genes are detected in the adult human brain, suggesting that these proteins are involved in the maintenance of the adult nervous system.1 BDNF and other neurotrophins are critically involved in long-term potentiation (LTP). BDNF-mediatedLTP is induced postsynaptically.2 BDNF has trophic effects on serotonergic (5-HT) neurons in the central nervoussystem.3 BDNF has an essential maintenance function in the regulation of anxiety-related behavior and in foodintake through central mediators in both the basal and fasted state.4 It plays a role in treating breathing disorderssuch as respiratory insufficiency after spinal injury.5 The mature form of BDNF is identical in all mammalsexamined, and the gene encoding human BDNF to chromosome 11, band p13. 6
Human Brain-derived neurotrophic factor Protein information
Uniprot ID BDNF_HUMAN
Uniprot AC Q9BYY7; Q9UC24;
UniGene Hs.502182;
GeneID 627
KEGG hsa:627;
Human Brain-derived neurotrophic factor Protein SEQUENCE
SEQUENCE 247 AA; 27818 MW; 0A60488254722A99 CRC64;
MTILFLTMVI SYFGCMKAAP MKEANIRGQG GLAYPGVRTH GTLESVNGPK
AGSRGLTSLA DTFEHVIEEL LDEDQKVRPN EENNKDADLY TSRVMLSSQV
PLEPPLLFLL EEYKNYLDAA NMSMRVRRHS DPARRGELSV CDSISEWVTA
ADKKTAVDMS GGTVTVLEKV PVSKGQLKQY FYETKCNPMG YTKEGCRGID
KRHWNSQCRT TQSYVRALTM DSKKRIGWRF IRIDTSCVCT LTIKRGR
UCSC uc001mrt.4; human. [P23560-1];

Human BDNF ELISA Kit

Human BDNF ELISA Kit;Human abrineurin ELISA Kit;Human ANON2 ELISA Kit;Human BULN2 ELISA Kit;Human brain derived neurotrophic factor ELISA Kit;Human brain-derived neurotrophic factor ELISA Kit;Human neurotrophin ELISA Kit;

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