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Mouse Bpgm ELISA Kit

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  • Alternative name

    Mouse BPGM ELISA Kit;Mouse 2,3-bisphosphoglycerate mutase, erythrocyte ELISA Kit;Mouse 2,3-bisphosphoglycerate synthase ELISA Kit;Mouse BPG-dependent PGAM ELISA Kit;Mouse AI323730 ELISA Kit;Mouse AL022789 ELISA Kit;Mouse C86192 ELISA Kit;Mouse 2,3-bisphosphoglycerate mutase ELISA Kit;Mouse bisphosphoglycerate mutase ELISA Kit;
  • Catalog
    E045881
  • species
    Mouse
  • GeneBpgm
  • Standard CurveMouse Bpgm ELISA Kit
  • Other Species Human BPGM ELISA KitBovine BPGM ELISA KitMonkey BPGM ELISA KitRabbit BPGM ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of BPGM. No significant cross-reactivity or interference between BPGM and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between BPGM and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity1.0 ng/mL.
  • Intended UseMouse Bpgm ELISA Kit allows for the in vitro quantitative determination of Bpgm , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Principle of the Assay: BPGM ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-BPGM antibody and an BPGM-HRP conjugate. The assay sample and buffer are incubated together with BPGM-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the BPGM concentration since BPGM from samples and BPGM-HRP conjugate compete for the anti-BPGM antibody binding site. Since the number of sites is limited, as more sites are occupied by BPGM from the sample, fewer sites are left to bind BPGM-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The BPGM concentration in each sample is interpolated from this standard curve.
  • Mouse Bisphosphoglycerate mutase Protein information
  • Uniprot ID PMGE_MOUSE
  • Uniprot AC P15327; Q543Z6;
  • UniGene Mm.282863;
  • GeneID 12183
  • KEGG mmu:12183;
  • Mouse Bisphosphoglycerate mutase Protein SEQUENCE

  • SEQUENCE 259 AA; 29978 MW; 6C2674005B41A0C5 CRC64;

    MSKHKLIILR HGEGQWNKEN RFCSWVDQKL NNDGLEEARN CGRQLKALNF

    EFDLVFTSIL NRSIHTAWLI LEELGQEWVP VESSWRLNER HYGALIGLNR

    EKMALNHGEE QVRLWRRSYN VTPPPIEESH PYFHEIYSDR RYKVCDVPLD

    QLPRSESLKD VLERLLPYWK ERIAPEILKG KSILISAHGN SSRALLKHLE

    GISDEDIINI TLPTGVPILL ELDENLRAVG PHQFLGNQEA IQAAIKKVDD

    QGKVKQGKQ

  • UCSC uc009bhf.1; mouse.;


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