Human BCL2L12 ELISA Kit

Alternative name
Human Bcl2-L-12 ELISA Kit;Human Bcl-2-related proline-rich protein ELISA Kit;Human BPR ELISA Kit;Human BCL2 like 12 ELISA Kit;Human bcl-2-like protein 12 ELISA Kit;Human BCL2-like 12 (proline rich) ELISA Kit;Human Bcl-2 related proline-rich protein ELISA Kit;
Catalog
E004583
species
Human
GeneBCL2L12
Standard Curve
SpecificityThis assay has high sensitivity and excellent specificity for detection of Bcl2L12 . No significant cross-reactivity or interference between Bcl2L12 and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity< 0.188ng/ml
Intended UseHuman BCL2L12 ELISA Kit allows for the in vitro quantitative determination of BCL2L12 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- Bcl2L12antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- Bcl2L12 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRPStreptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Bcl2L12 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Bcl2L12 can be calculated.
Human Bcl-2-like protein 12 Protein information
Uniprot ID B2L12_HUMAN
Uniprot AC Q9HB09; Q3SY11; Q3SY13; Q96I96; Q9HB08;
UniGene Hs.289052;
GeneID 83596
KEGG hsa:83596;
Human Bcl-2-like protein 12 Protein SEQUENCE
SEQUENCE 334 AA; 36821 MW; 5398E54C83E7CAB7 CRC64;
MGRPAGLFPP LCPFLGFRPE ACWERHMQIE RAPSVPPFLR WAGYRPGPVR
RRGKVELIKF VRVQWRRPQV EWRRRRWGPG PGASMAGSEE LGLREDTLRV
LAAFLRRGEA AGSPVPTPPR SPAQEEPTDF LSRLRRCLPC SLGRGAAPSE
SPRPCSLPIR PCYGLEPGPA TPDFYALVAQ RLEQLVQEQL KSPPSPELQG
PPSTEKEAIL RRLVALLEEE AEVINQKLAS DPALRSKLVR LSSDSFARLV
ELFCSRDDSS RPSRACPGPP PPSPEPLARL ALAMELSRRV AGLGGTLAGL
SVEHVHSFTP WIQAHGGWEG ILAVSPVDLN LPLD
UCSC uc002ppa.4; human. [Q9HB09-1];

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Category

Human BCL2L12 ELISA Kit

Human Bcl2-L-12 ELISA Kit;Human Bcl-2-related proline-rich protein ELISA Kit;Human BPR ELISA Kit;Human BCL2 like 12 ELISA Kit;Human bcl-2-like protein 12 ELISA Kit;Human BCL2-like 12 (proline rich) ELISA Kit;Human Bcl-2 related proline-rich protein ELISA Kit;