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Mouse Tectb ELISA Kit

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  • Alternative name

    Mouse Tctnb ELISA Kit;Mouse tectorin beta ELISA Kit;Mouse beta-tectorin ELISA Kit;Mouse [b]-tectorin ELISA Kit;
  • Catalog
    E045771
  • species
    Mouse
  • GeneTectb
  • Standard CurveMouse Tectb ELISA Kit
  • Other Species Human TECTB ELISA KitChicken TECTB ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of TECTB. No significant cross-reactivity or interference between TECTB and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between TECTB and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity1.0 ng/mL.
  • Intended UseMouse Tectb ELISA Kit allows for the in vitro quantitative determination of Tectb , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Categories/FamilyNeuroscience
  • Product Description
    specifical    Principle of the assay: TECTB ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-TECTB antibody and an TECTB-HRP conjugate. The assay sample and buffer are incubated together with TECTB-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the TECTB concentration since TECTB from samples and TECTB-HRP conjugate compete for the anti-TECTB antibody binding site. Since the number of sites is limited, as more sites are occupied by TECTB from the sample, fewer sites are left to bind TECTB-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The TECTB concentration in each sample is interpolated from this standard curve.
  • Mouse Beta-tectorin Protein information
  • Uniprot ID TECTB_MOUSE
  • Uniprot AC O08524;
  • UniGene Mm.42139;
  • GeneID 21684
  • KEGG mmu:21684;
  • Mouse Beta-tectorin Protein SEQUENCE

  • SEQUENCE 329 AA; 36986 MW; CC69A33EBC270AB3 CRC64;

    MVVRAFVLLA LFAEASAKSC TPNKADVILV FCYPKTIITK IPECPYGWEV

    HQLALGGLCY NGVHEGGYYQ FVIPDLSPKN KSYCGTQSEY KPPIYHFYSH

    IVSNDSTVIV KNQPVNYSFS CTYHSTYLVN QAAFDQRVAT VHVKNGSMGT

    FESQLSLNFY TNAKFSTKKE APFVLETSEI GSDLFAGVEA KGLSVRFKVV

    LNSCWATPSA DFMYPLQWQL INKGCPTDET VLVHENGKDH RATFQFNAFR

    FQNIPKLSKV WLHCETFICD SEKLSCPVNC DKRKRMLRDQ TGGVLVVELS

    LRSRAFSGLC DFSDVLLHLI LMLGTWAVL

  • UCSC uc008hxm.1; mouse.;



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