Mouse Defb3 ELISA Kit

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  • Alternative name

    Mouse BD-3 ELISA Kit;Mouse mBD-3 ELISA Kit;Mouse Defensin, beta 3 ELISA Kit;Mouse Bd3 ELISA Kit;Mouse defensin beta 3 ELISA Kit;Mouse beta-defensin 3 ELISA Kit;

  • Catalog
    E045700
  • species
    Mouse
  • GeneDefb3
  • Other Species Bovine DEFB3 ELISA KitRat Defb3 ELISA Kit
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity<0.094ng/ml
  • Intended UseMouse Defb3 ELISA Kit allows for the in vitro quantitative determination of Defb3 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Mouse Defb3 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Mouse Defb3 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Mouse Defb3 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Mouse Defb3 can be calculated.
  • Mouse Beta-defensin 3 Protein information
  • Uniprot ID DEFB3_MOUSE
  • Uniprot AC Q9WTL0; Q29R78;
  • UniGene Mm.103651;
  • GeneID 27358
  • KEGG mmu:27358;
  • Mouse Beta-defensin 3 Protein SEQUENCE
  • SEQUENCE 63 AA; 7126 MW; 9D59BC8AD16EA330 CRC64;

    MRIHYLLFAF LLVLLSPPAA FSKKINNPVS CLRKGGRCWN RCIGNTRQIG

    SCGVPFLKCC KRK

  • UCSC uc009lao.1; mouse.;



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