Mouse B4galt5 ELISA Kit

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  • Alternative name

    Mouse beta-1,4-GalTase 5 ELISA Kit;Mouse beta4Gal-T5 ELISA Kit;Mouse b4Gal-T5 ELISA Kit;Mouse beta-1,4-GalT II ELISA Kit;Mouse UDP-Gal:beta-GlcNAc beta-1,4-galactosyltransferase 5 ELISA Kit;Mouse UDP-galactose:beta-N-acetylglucosamine beta-1,4-galactosyltransferase 5 ELISA Kit;Mouse Bgt-5 ELISA Kit;Mouse 9430078I07Rik ELISA Kit;Mouse AW049941 ELISA Kit;Mouse AW539721 ELISA Kit;Mouse UDP-Gal:betaGlcNAc beta 1,4-galactosyltransferase, polypeptide 5 ELISA Kit;Mouse beta-1,4-galactosyltransferase 5 ELISA Kit;Mouse beta-1,4-galactosyltransferase V ELISA Kit;

  • Catalog
    E045640
  • species
    Mouse
  • GeneB4galt5
  • Standard CurveMouse B4galt5 ELISA Kit
  • Other Species Human B4GALT5 ELISA Kit
  • SpecificityThis assay recognizes recombinant and natural Mouse b4Gal-T5. No significant cross-reactivity or interference was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Intended UseMouse B4galt5 ELISA Kit allows for the in vitro quantitative determination of B4galt5 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay: The ELISA is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to b4Gal-T5, During the reaction, b4Gal-T5 in the sample or standard competes with a fixed amount of biotin-labeled b4Gal-T5 for sites on a pre-coated Monoclonal antibody specific to b4Gal-T5. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of b4Gal-T5 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Mouse Beta-1,4-galactosyltransferase 5 Protein information
  • Uniprot ID B4GT5_MOUSE
  • Uniprot AC Q9JMK0; A2A5T7;
  • UniGene Mm.200886; Mm.489868;
  • GeneID 56336
  • KEGG mmu:56336;
  • Mouse Beta-1,4-galactosyltransferase 5 Protein SEQUENCE
  • SEQUENCE 388 AA; 44814 MW; FB3D6FBA2F8B3775 CRC64;

    MRARRGLLRL PRRSLLAALF FFSLSSSLLY FVYVAPGIVN TYLFMVQAQG

    ILLRDNVRTI GAQVYEQVVR SAYAKRNSSL NDSDYPLDLN HSEAFPPTTT

    FLPEDFTYFA NHPCPERLPS MKGPIDINMS EIAMDDIHEL FSRDPAIKLG

    GHWKPADCVP RWKVAILIPF RNRHEHLPVL LRHLLPMLQR QRLQFAFYVI

    EQVGTQPFNR AMLFNVGFQE AMKDLDWDCL IFHDVDHIPE SDRNYYGCGQ

    MPRHFATKLD KYMYLLPYTE FFGGVSGLTV EQFRKINGFP NAFWGWGGED

    DDLWNRVQNA GYSVSRPEGD TGKYKSIPHH HRGEVQFLGR YALLRKSKER

    QGLDGLNNLN YSANVTYDAL YKNITVNLTP ELAQVTEY

  • UCSC uc008nzm.1; mouse.;




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