Mouse B4galt5 ELISA Kit

Alternative name
Mouse beta-1,4-GalTase 5 ELISA Kit;Mouse beta4Gal-T5 ELISA Kit;Mouse b4Gal-T5 ELISA Kit;Mouse beta-1,4-GalT II ELISA Kit;Mouse UDP-Gal:beta-GlcNAc beta-1,4-galactosyltransferase 5 ELISA Kit;Mouse UDP-galactose:beta-N-acetylglucosamine beta-1,4-galactosyltransferase 5 ELISA Kit;Mouse Bgt-5 ELISA Kit;Mouse 9430078I07Rik ELISA Kit;Mouse AW049941 ELISA Kit;Mouse AW539721 ELISA Kit;Mouse UDP-Gal:betaGlcNAc beta 1,4-galactosyltransferase, polypeptide 5 ELISA Kit;Mouse beta-1,4-galactosyltransferase 5 ELISA Kit;Mouse beta-1,4-galactosyltransferase V ELISA Kit;
Catalog
E045640
species
Mouse
GeneB4galt5
Standard Curve
Other Species Human B4GALT5 ELISA Kit
SpecificityThis assay recognizes recombinant and natural Mouse b4Gal-T5. No significant cross-reactivity or interference was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Intended UseMouse B4galt5 ELISA Kit allows for the in vitro quantitative determination of B4galt5 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: The ELISA is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to b4Gal-T5, During the reaction, b4Gal-T5 in the sample or standard competes with a fixed amount of biotin-labeled b4Gal-T5 for sites on a pre-coated Monoclonal antibody specific to b4Gal-T5. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of b4Gal-T5 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Mouse Beta-1,4-galactosyltransferase 5 Protein information
Uniprot ID B4GT5_MOUSE
Uniprot AC Q9JMK0; A2A5T7;
UniGene Mm.200886; Mm.489868;
GeneID 56336
KEGG mmu:56336;
Mouse Beta-1,4-galactosyltransferase 5 Protein SEQUENCE
SEQUENCE 388 AA; 44814 MW; FB3D6FBA2F8B3775 CRC64;
MRARRGLLRL PRRSLLAALF FFSLSSSLLY FVYVAPGIVN TYLFMVQAQG
ILLRDNVRTI GAQVYEQVVR SAYAKRNSSL NDSDYPLDLN HSEAFPPTTT
FLPEDFTYFA NHPCPERLPS MKGPIDINMS EIAMDDIHEL FSRDPAIKLG
GHWKPADCVP RWKVAILIPF RNRHEHLPVL LRHLLPMLQR QRLQFAFYVI
EQVGTQPFNR AMLFNVGFQE AMKDLDWDCL IFHDVDHIPE SDRNYYGCGQ
MPRHFATKLD KYMYLLPYTE FFGGVSGLTV EQFRKINGFP NAFWGWGGED
DDLWNRVQNA GYSVSRPEGD TGKYKSIPHH HRGEVQFLGR YALLRKSKER
QGLDGLNNLN YSANVTYDAL YKNITVNLTP ELAQVTEY
UCSC uc008nzm.1; mouse.;

Category

Mouse B4galt5 ELISA Kit