Mouse Gcnt3 ELISA Kit

Alternative name
Mouse C2GnT-mucin type ELISA Kit;Mouse C2GnT-M ELISA Kit;Mouse mucus-type core 2 beta-1,6-N-acetylglucosaminyltransferase ELISA Kit;Mouse 2010013H22Rik ELISA Kit;Mouse 2210021I22Rik ELISA Kit;Mouse 2210401J11Rik ELISA Kit;Mouse glucosaminyl (N-acetyl) transferase 3, mucin type ELISA Kit;Mouse beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase 3 ELISA Kit;Mouse core 2 beta-1,6-N-acetylglucosaminyltransferase II ELISA Kit;
Catalog
E045614
species
Mouse
GeneGcnt3
SpecificityThis assay has high sensitivity and excellent specificity for detection of GCNT3. No significant cross-reactivity or interference between GCNT3 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between GCNT3 and all the analogues, therefore, cross reaction may still exist in some cases.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity0.1 ng/mL.
Intended UseMouse Gcnt3 ELISA Kit allows for the in vitro quantitative determination of Gcnt3 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Categories/FamilyCell Biology
Product Description
specificalIntended Uses: This GCNT3 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse GCNT3. This ELISA kit for research use only, not for therapeutic or diagnostic applications! Principle of the Assay||GCNT3 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-GCNT3 antibody and an GCNT3-HRP conjugate. The assay sample and buffer are incubated together with GCNT3-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the GCNT3 concentration since GCNT3 from samples and GCNT3-HRP conjugate compete for the anti-GCNT3 antibody binding site. Since the number of sites is limited, as more sites are occupied by GCNT3 from the sample, fewer sites are left to bind GCNT3-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The GCNT3 concentration in each sample is interpolated from this standard curve.
Mouse Beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase 3 Protein information
Uniprot ID GCNT3_MOUSE
Uniprot AC Q5JCT0; A2TIK7; Q8VCX9; Q9D8A3;
UniGene Mm.195555;
GeneID 72077
KEGG mmu:72077;
Mouse Beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase 3 Protein SEQUENCE
SEQUENCE 437 AA; 50698 MW; FF0C1EC8F25320F1 CRC64;
MTSWQRLCWH YRLWTLGCYM LLAILALKLS LRLKCDFDAM DLDSEEFQSQ
YCRDLLYKTL KLPAKSSINC SGVIRGEQKA VTQALLNNLE IKKKQQLFTE
ADYLRMTADC EHFKTKRKFI QVPLSKEEAS FPIAYSMVVH EKIENFERLL
RAVYTPQNVY CVHMDQKSSE PFKQAVRAIV SCFPNVFIAS KLVSVVYASW
SRVQADLNCM EDLLQSPVPW KYLLNTCGTD FPIKTNAEMV KALKLLKGQN
SMESEVPPPH KKSRWKYHYE VTDTLHMTSK RKTPPPNNLT MFTGNAYMVA
SRDFIEHVFS NSKARQLIEW VKDTYSPDEH LWATLQRASW MPGSDPLHRK
FDLSDMRAIA RLTKWYDHEG DIENGAPYTS CSGIHQRAVC VYGSGDLHWI
LQNHHLLANK FDPKVDDNVL QCLEEYLRHK AIYGTEL
UCSC uc009qnt.1; mouse.;

Category

Mouse Gcnt3 ELISA Kit