Mouse Gcnt3 ELISA Kit

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  • Alternative name

    Mouse C2GnT-mucin type ELISA Kit;Mouse C2GnT-M ELISA Kit;Mouse mucus-type core 2 beta-1,6-N-acetylglucosaminyltransferase ELISA Kit;Mouse 2010013H22Rik ELISA Kit;Mouse 2210021I22Rik ELISA Kit;Mouse 2210401J11Rik ELISA Kit;Mouse glucosaminyl (N-acetyl) transferase 3, mucin type ELISA Kit;Mouse beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase 3 ELISA Kit;Mouse core 2 beta-1,6-N-acetylglucosaminyltransferase II ELISA Kit;

  • Catalog
    E045614
  • species
    Mouse
  • GeneGcnt3
  • Standard CurveMouse Gcnt3 ELISA Kit
  • Other Species Human GCNT3 ELISA KitBovine GCNT3 ELISA KitRat Gcnt3 ELISA KitSheep GCNT3 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of GCNT3. No significant cross-reactivity or interference between GCNT3 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between GCNT3 and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.1 ng/mL.
  • Intended UseMouse Gcnt3 ELISA Kit allows for the in vitro quantitative determination of Gcnt3 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Categories/FamilyCell Biology
  • Product Description
    specifical
    Intended Uses: This GCNT3 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse GCNT3. This ELISA kit for research use only, not for therapeutic or diagnostic applications! Principle of the Assay||GCNT3 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-GCNT3 antibody and an GCNT3-HRP conjugate. The assay sample and buffer are incubated together with GCNT3-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the GCNT3 concentration since GCNT3 from samples and GCNT3-HRP conjugate compete for the anti-GCNT3 antibody binding site. Since the number of sites is limited, as more sites are occupied by GCNT3 from the sample, fewer sites are left to bind GCNT3-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The GCNT3 concentration in each sample is interpolated from this standard curve.
  • Mouse Beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase 3 Protein information
  • Uniprot ID GCNT3_MOUSE
  • Uniprot AC Q5JCT0; A2TIK7; Q8VCX9; Q9D8A3;
  • UniGene Mm.195555;
  • GeneID 72077
  • KEGG mmu:72077;
  • Mouse Beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase 3 Protein SEQUENCE
  • SEQUENCE 437 AA; 50698 MW; FF0C1EC8F25320F1 CRC64;

    MTSWQRLCWH YRLWTLGCYM LLAILALKLS LRLKCDFDAM DLDSEEFQSQ

    YCRDLLYKTL KLPAKSSINC SGVIRGEQKA VTQALLNNLE IKKKQQLFTE

    ADYLRMTADC EHFKTKRKFI QVPLSKEEAS FPIAYSMVVH EKIENFERLL

    RAVYTPQNVY CVHMDQKSSE PFKQAVRAIV SCFPNVFIAS KLVSVVYASW

    SRVQADLNCM EDLLQSPVPW KYLLNTCGTD FPIKTNAEMV KALKLLKGQN

    SMESEVPPPH KKSRWKYHYE VTDTLHMTSK RKTPPPNNLT MFTGNAYMVA

    SRDFIEHVFS NSKARQLIEW VKDTYSPDEH LWATLQRASW MPGSDPLHRK

    FDLSDMRAIA RLTKWYDHEG DIENGAPYTS CSGIHQRAVC VYGSGDLHWI

    LQNHHLLANK FDPKVDDNVL QCLEEYLRHK AIYGTEL

  • UCSC uc009qnt.1; mouse.;



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