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Mouse Tgfbi ELISA Kit

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  • Alternative name

    Mouse beta ig-h3 ELISA Kit;Mouse 68kDa ELISA Kit;Mouse AI181842 ELISA Kit;Mouse AI747162 ELISA Kit;Mouse Beta-ig ELISA Kit;Mouse big-h3 ELISA Kit;Mouse transforming growth factor, beta induced ELISA Kit;Mouse transforming growth factor-beta-induced protein ig-h3 ELISA Kit;Mouse transforming growth factor, beta induced, 68 kDa ELISA Kit;
  • Catalog
    E045604
  • species
    Mouse
  • GeneTgfbi
  • SpecificityNatural and recombinant mouse beta IG-H3
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity Sample For quantitative detection of mouse beta IG-H3 in cell culture supernates, serum and
  • Intended UseMouse Tgfbi ELISA Kit allows for the in vitro quantitative determination of Tgfbi , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Principle of the assay: Mouse beta IG-H3 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for beta IG-H3 has been precoated onto 96-well plates. Standards(NSO, G24-H683) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for beta IG-H3 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse beta IG-H3 amount of sample captured in plate. Background: Transforming growth factor, beta-induced, 68kDa, also known as TGFBI (initially called BIGH3, BIG-H3), is a protein which in humans is encoded by the TGFBI gene. It is mapped to 5q31.1. This gene encodes an RGD-containing protein that binds to type I, II and IV collagens. The RGD motif is found in many extracellular matrix proteins modulating cell adhesion and serves as a ligand recognition sequence for several integrins. This protein plays a role in cell-collagen interactions and may be involved in endochondrial bone formation in cartilage. The protein is induced by transforming growth factor-beta and acts to inhibit cell adhesion.
  • Mouse Transforming growth factor-beta-induced protein ig-h3 Protein information
  • Uniprot ID BGH3_MOUSE
  • Uniprot AC P82198; Q3U9R1;
  • UniGene Mm.14455;
  • GeneID 21810
  • KEGG mmu:21810;
  • Mouse Transforming growth factor-beta-induced protein ig-h3 Protein SEQUENCE

  • SEQUENCE 683 AA; 74597 MW; 2B6E9DBB9C50F52B CRC64;

    MALLMRLLTL ALALSVGPAG TLAGPAKSPY QLVLQHSRLR GRQHGPNVCA

    VQKVIGTNKK YFTNCKQWYQ RKICGKSTVI SYECCPGYEK VPGEKGCPAA

    LPLSNLYETM GVVGSTTTQL YTDRTEKLRP EMEGPGSFTI FAPSNEAWSS

    LPAEVLDSLV SNVNIELLNA LRYHMVDRRV LTDELKHGMT LTSMYQNSNI

    QIHHYPNGIV TVNCARLLKA DHHATNGVVH LIDKVISTIT NNIQQIIEIE

    DTFETLRAAV AASGLNTVLE GDGQFTLLAP TNEAFEKIPA ETLNRILGDP

    EALRDLLNNH ILKSAMCAEA IVAGMSMETL GGTTLEVGCS GDKLTINGKA

    VISNKDILAT NGVIHFIDEL LIPDSAKTLL ELAGESDVST AIDILKQAGL

    DTHLSGKEQL TFLAPLNSVF KDGVPRIDAQ MKTLLLNHMV KEQLASKYLY

    SGQTLDTLGG KKLRVFVYRN SLCIENSCIA AHDKRGRFGT LFTMDRMLTP

    PMGTVMDVLK GDNRFSMLVA AIQSAGLMEI LNREGVYTVF APTNEAFQAM

    PPEELNKLLA NAKELTNILK YHIGDEILVS GGIGALVRLK SLQGDKLEVS

    SKNNVVSVNK EPVAETDIMA TNGVVYAINT VLQPPANRPQ ERGDELADSA

    LEIFKQASAY SRAAQRSVRL APVYQRLLER MKH

  • UCSC uc011zae.1; mouse.;



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