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Alternative name
Beta-defensin 103 ELISA KIT; Beta-defensin 3 ELISA KIT; BD-3 ELISA KIT; DEFB-3 ELISA KIT; HBD3 ELISA KIT; hBD-3 ELISA KIT; Defensin, beta 103 ELISA KIT; Defensin-like proteinDEFB103A ELISA KIT; BD3 ELISA KIT; DEFB103 ELISA KIT; DEFB3
DEFB103B ELISA KIT; BD-3 ELISA KIT; DEFB-3 ELISA KIT; HBD3 ELISA KIT; hBD-3 ELISA KIT
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Catalog
E045596
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species
Mouse
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GeneBDF
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Standard Curve
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Other Species
Human BDF ELISA KitMouse BDF-1 ELISA Kit
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SpecificityThis assay has high sensitivity and excellent specificity for detection of betaDEF. No significant cross-reactivity or interference between betaDEF and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between betaDEF and all the analogues, therefore, cross reaction may still exist in some cases.
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SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
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Sensitivity0.1 ng/mL.
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Intended UseMouse BDF ELISA Kit allows for the in vitro quantitative determination of BDF , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
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StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
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Product Categories/FamilyImmunology
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Product Description
specificalIntended Uses: This betaDEF ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse betaDEF. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
Principle of the Assay||betaDEF ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-betaDEF antibody and an betaDEF-HRP conjugate. The assay sample and buffer are incubated together with betaDEF-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the betaDEF concentration since betaDEF from samples and betaDEF-HRP conjugate compete for the anti-betaDEF antibody binding site. Since the number of sites is limited, as more sites are occupied by betaDEF from the sample, fewer sites are left to bind betaDEF-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The betaDEF concentration in each sample is interpolated from this standard curve.
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