Mouse Btc ELISA Kit

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  • Alternative name

    Mouse BTC ELISA Kit;Mouse Bcn ELISA Kit;Mouse betacellulin, epidermal growth factor family member ELISA Kit;Mouse betacellulin ELISA Kit;Mouse probetacellulin ELISA Kit;

  • Catalog
    E045593
  • species
    Mouse
  • GeneBtc
  • Other Species Human BTC ELISA KitBovine BTC ELISA Kit
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity< 4.69pg/ml
  • Intended UseMouse Btc ELISA Kit allows for the in vitro quantitative determination of Btc , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Mouse BTC antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Mouse BTC antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Mouse BTC amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Mouse BTC can be calculated.
  • Mouse Betacellulin Protein information
  • Uniprot ID BTC_MOUSE
  • Uniprot AC Q05928;
  • UniGene Mm.2024;
  • GeneID 12223
  • KEGG mmu:12223;
  • Mouse Betacellulin Protein SEQUENCE
  • SEQUENCE 177 AA; 19664 MW; 066BB34F0E13F82B CRC64;

    MDPTAPGSSV SSLPLLLVLA LGLAILHCVV ADGNTTRTPE TNGSLCGAPG

    ENCTGTTPRQ KVKTHFSRCP KQYKHYCIHG RCRFVVDEQT PSCICEKGYF

    GARCERVDLF YLQQDRGQIL VVCLIVVMVV FIILVIGVCT CCHPLRKHRK

    KKKEEKMETL DKDKTPISED IQETNIA

  • UCSC uc008ybv.2; mouse.;



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