Mouse BAFF ELISA Kit

Contact Us
  • Alternative name

    Tumor necrosis factor ligand superfamily member 13B ELISA KIT; B lymphocyte stimulator ELISA KIT; BLySTNFSF13B ELISA KIT; BAFF ELISA KIT; BLYS ELISA KIT; TALL1 ELISA KIT; TNFSF20 ELISA KIT; ZTNF4 ELISA KIT; BLyS ELISA KIT; TALL-1 ELISA KIT

  • Catalog
    E045350
  • species
    Mouse
  • GeneBAFF
  • Standard CurveMouse BAFF ELISA Kit
  • Other Species Human BAFF-R ELISA KitHuman BAFF ELISA KitHuman BAFF/CD257 ELISA KitMouse BAFF/CD257 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of B-Cell Activating Factor (BAFF). No significant cross-reactivity or interference between B-Cell Activating Factor (BAFF) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 26.1pg/mL.
  • Intended UseMouse BAFF ELISA Kit allows for the in vitro quantitative determination of BAFF , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageFor 5-7days:Store the whole kit at 4℃
    For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
  • Product Description
    specifical
    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to B-Cell Activating Factor (BAFF). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to B-Cell Activating Factor (BAFF). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain B-Cell Activating Factor (BAFF), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of B-Cell Activating Factor (BAFF) in the samples is then determined by comparing the O.D. of the samples to the standard curve.




tag

Inquiry About Mouse BAFF ELISA Kit If you hope to order it or contact us directly, please contact us via sales@biobool.com. We will get back to you in 12hrs,Thanks!