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Alternative name
Tumor necrosis factor ligand superfamily member 13B ELISA KIT; B lymphocyte stimulator ELISA KIT; BLySTNFSF13B ELISA KIT; BAFF ELISA KIT; BLYS ELISA KIT; TALL1 ELISA KIT; TNFSF20 ELISA KIT; ZTNF4 ELISA KIT; BLyS ELISA KIT; TALL-1 ELISA KIT
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Catalog
E045349
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species
Mouse
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GeneBAFF/CD257
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SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
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Sensitivity< 37.5pg/ml
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Intended UseMouse BAFF/CD257 ELISA Kit allows for the in vitro quantitative determination of BAFF/CD257 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
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StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
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Product Description
specificalPrinciple of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Mouse BAFF antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Mouse BAFF antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Mouse BAFF amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Mouse BAFF can be calculated.
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