Mouse Axin1 ELISA Kit

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  • Alternative name

    Mouse axis inhibition protein 1 ELISA Kit;Mouse protein Fused ELISA Kit;Mouse Axin ELISA Kit;Mouse Fu ELISA Kit;Mouse AI316800 ELISA Kit;Mouse Kb ELISA Kit;Mouse Ki ELISA Kit;Mouse fused ELISA Kit;Mouse kinky ELISA Kit;Mouse knobbly ELISA Kit;Mouse axin 1 ELISA Kit;Mouse axin-1 ELISA Kit;

  • Catalog
    E045318
  • species
    Mouse
  • GeneAxin1
  • Standard CurveMouse Axin1 ELISA Kit
  • Other Species Human AXIN1 ELISA KitChicken AXIN1 ELISA KitRat Axin1 ELISA Kit
  • SpecificityThis assay recognizes recombinant and natural mouse Axin-1. No significant cross-reactivity or interference was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Intended UseMouse Axin1 ELISA Kit allows for the in vitro quantitative determination of Axin1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to Axin-1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Axin-1 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Axin-1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of Axin-1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Mouse Axin-1 Protein information
  • Uniprot ID AXIN1_MOUSE
  • Uniprot AC O35625;
  • UniGene Mm.23684;
  • GeneID 12005
  • KEGG mmu:12005;
  • Mouse Axin-1 Protein SEQUENCE
  • SEQUENCE 863 AA; 96276 MW; 2216D66E92387B81 CRC64;

    MNVQEQGFPL DLGASFTEDA PRPPVPGEEG ELVSTDSRPV NHSFCSGKGT

    SIKSETSTAT PRRSDLDLGY EPEGSASPTP PYLRWAESLH SLLDDQDGIS

    LFRTFLKQEG CADLLDFWFA CSGFRKLEPC DSNEEKRLKL ARAIYRKYIL

    DSNGIVSRQT KPATKSFIKD CVMKQQIDPA MFDQAQTEIQ STMEENTYPS

    FLKSDIYLEY TRTGSESPKV CSDQSSGSGT GKGMSGYLPT LNEDEEWKCD

    QDADEDDGRD PLPPSRLTQK LLLETAAPRA PSSRRYNEGR ELRYGSWREP

    VNPYYVNSGY ALAPATSAND SEQQSLSSDA DTLSLTDSSV DGIPPYRIRK

    QHRREMQESI QVNGRVPLPH IPRTYRMPKE IRVEPQKFAE ELIHRLEAVQ

    RTREAEEKLE ERLKRVRMEE EGEDGEMPSG PMASHKLPSV PAWHHFPPRY

    VDMGCSGLRD AHEENPESIL DEHVQRVMRT PGCQSPGPGH RSPDSGHVAK

    TAVLGGTASG HGKHVPKLGL KLDTAGLHHH RHVHHHVHHN SARPKEQMEA

    EVARRVQSSF SWGPETHGHA KPRSYSENAG TTLSAGDLAF GGKTSAPSKR

    NTKKAESGKN ANAEVPSTTE DAEKNQKIMQ WIIEGEKEIS RHRKAGHGSS

    GLRKQQAHES SRPLSIERPG AVHPWVSAQL RNSVQPSHLF IQDPTMPPNP

    APNPLTQLEE ARRRLEEEEK RANKLPSKQR YVQAVMQRGR TCVRPACAPV

    LSVVPAVSDL ELSETETKSQ RKAGGGSAPP CDSIVVAYYF CGEPIPYRTL

    VRGRAVTLGQ FKELLTKKGS YRYYFKKVSD EFDCGVVFEE VREDEAVLPV

    FEEKIIGKVE KVD



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