Mouse Atp1b3 ELISA Kit

Alternative name
Mouse sodium/potassium-dependent ATPase subunit beta-3 ELISA Kit;Mouse ATPB-3 ELISA Kit;Mouse AA409958 ELISA Kit;Mouse AI664000 ELISA Kit;Mouse AW212096 ELISA Kit;Mouse ATPase, Na+/K+ transporting, beta 3 polypeptide ELISA Kit;Mouse sodium/potassium-transporting ATPase subunit beta-3 ELISA Kit;Mouse ATPase, Na+/K+ beta 3 polypeptide ELISA Kit;Mouse Na K-ATPase beta-3 subunit ELISA Kit;
Catalog
E045255
species
Mouse
GeneAtp1b3
SpecificityThis assay has high sensitivity and excellent specificity for detection of ATPase, Na+/K+ Transporting Beta 3 Polypeptide (ATP1b3). No significant cross-reactivity or interference between ATPase, Na+/K+ Transporting Beta 3 Polypeptide (ATP1b3) and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityTypically less than 0.066ng/mL.
Intended UseMouse Atp1b3 ELISA Kit allows for the in vitro quantitative determination of Atp1b3 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to ATPase, Na+/K+ Transporting Beta 3 Polypeptide (ATP1b3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to ATPase, Na+/K+ Transporting Beta 3 Polypeptide (ATP1b3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain ATPase, Na+/K+ Transporting Beta 3 Polypeptide (ATP1b3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of ATPase, Na+/K+ Transporting Beta 3 Polypeptide (ATP1b3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Mouse Sodium/potassium-transporting ATPase subunit beta-3 Protein information
Uniprot ID AT1B3_MOUSE
Uniprot AC P97370;
UniGene Mm.424;
GeneID 11933
KEGG mmu:11933;
Mouse Sodium/potassium-transporting ATPase subunit beta-3 Protein SEQUENCE
SEQUENCE 278 AA; 31776 MW; 98A46FF6EDA85AAC CRC64;
MTKTEKKSFH QSLAEWKLFI YNPSSGEFLG RTSKSWGLIL LFYLVFYGFL
AALFTFTMWA MLQTLNDEVP KYRDQIPSPG LMVFPKPQTA LEYTFSMSEP
QTYKKLVEDL ESFLKPYSVE EQKNLTSCPD GAPFIQHGPD YRACQFPVSL
LEECSGVTDA NFGYSKGQPC ILVKMNRIID LIPDGYPQIS CLPKEENATI
ATYPEFGVLD LKYFPYYGKK RHVGYRQPLV AVQVKFDSGL NKKEVTVECH
IAGTRNLKNK NERDKFLGRV SFKVTARA
UCSC uc009rck.2; mouse.;

Mouse Atp1b3 ELISA Kit

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