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Mouse Atp1b3 ELISA Kit

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  • Alternative name

    Mouse sodium/potassium-dependent ATPase subunit beta-3 ELISA Kit;Mouse ATPB-3 ELISA Kit;Mouse AA409958 ELISA Kit;Mouse AI664000 ELISA Kit;Mouse AW212096 ELISA Kit;Mouse ATPase, Na+/K+ transporting, beta 3 polypeptide ELISA Kit;Mouse sodium/potassium-transporting ATPase subunit beta-3 ELISA Kit;Mouse ATPase, Na+/K+ beta 3 polypeptide ELISA Kit;Mouse Na K-ATPase beta-3 subunit ELISA Kit;
  • Catalog
    E045255
  • species
    Mouse
  • GeneAtp1b3
  • Standard CurveMouse Atp1b3 ELISA Kit
  • Other Species Human ATP1B3 ELISA KitBovine ATP1B3 ELISA KitGuinea Pig ATP1B3 ELISA KitChicken ATP1B3 ELISA KitRabbit ATP1B3 ELISA KitRat Atp1b3 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of ATPase, Na+/K+ Transporting Beta 3 Polypeptide (ATP1b3). No significant cross-reactivity or interference between ATPase, Na+/K+ Transporting Beta 3 Polypeptide (ATP1b3) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.066ng/mL.
  • Intended UseMouse Atp1b3 ELISA Kit allows for the in vitro quantitative determination of Atp1b3 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to ATPase, Na+/K+ Transporting Beta 3 Polypeptide (ATP1b3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to ATPase, Na+/K+ Transporting Beta 3 Polypeptide (ATP1b3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain ATPase, Na+/K+ Transporting Beta 3 Polypeptide (ATP1b3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of ATPase, Na+/K+ Transporting Beta 3 Polypeptide (ATP1b3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Mouse Sodium/potassium-transporting ATPase subunit beta-3 Protein information
  • Uniprot ID AT1B3_MOUSE
  • Uniprot AC P97370;
  • UniGene Mm.424;
  • GeneID 11933
  • KEGG mmu:11933;
  • Mouse Sodium/potassium-transporting ATPase subunit beta-3 Protein SEQUENCE

  • SEQUENCE 278 AA; 31776 MW; 98A46FF6EDA85AAC CRC64;

    MTKTEKKSFH QSLAEWKLFI YNPSSGEFLG RTSKSWGLIL LFYLVFYGFL

    AALFTFTMWA MLQTLNDEVP KYRDQIPSPG LMVFPKPQTA LEYTFSMSEP

    QTYKKLVEDL ESFLKPYSVE EQKNLTSCPD GAPFIQHGPD YRACQFPVSL

    LEECSGVTDA NFGYSKGQPC ILVKMNRIID LIPDGYPQIS CLPKEENATI

    ATYPEFGVLD LKYFPYYGKK RHVGYRQPLV AVQVKFDSGL NKKEVTVECH

    IAGTRNLKNK NERDKFLGRV SFKVTARA

  • UCSC uc009rck.2; mouse.;


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