Mouse Atp7b ELISA Kit

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  • Alternative name

    Mouse copper pump 2 ELISA Kit;Mouse wilson disease-associated protein homolog ELISA Kit;Mouse WND ELISA Kit;Mouse Atp7a ELISA Kit;Mouse tx ELISA Kit;Mouse ATPase, Cu++ transporting, beta polypeptide ELISA Kit;Mouse copper-transporting ATPase 2 ELISA Kit;Mouse Wilson protein ELISA Kit;Mouse toxic milk ELISA Kit;

  • Catalog
    E045220
  • species
    Mouse
  • GeneAtp7b
  • Standard CurveMouse Atp7b ELISA Kit
  • Other Species Human ATP7B ELISA KitRat Atp7b ELISA KitSheep ATP7B ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of ATPase, Cu++ Transporting Beta Polypeptide (ATP7b). No significant cross-reactivity or interference between ATPase, Cu++ Transporting Beta Polypeptide (ATP7b) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.055ng/mL.
  • Intended UseMouse Atp7b ELISA Kit allows for the in vitro quantitative determination of Atp7b , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to ATPase, Cu++ Transporting Beta Polypeptide (ATP7b). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to ATPase, Cu++ Transporting Beta Polypeptide (ATP7b). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain ATPase, Cu++ Transporting Beta Polypeptide (ATP7b), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of ATPase, Cu++ Transporting Beta Polypeptide (ATP7b) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Mouse Copper-transporting ATPase 2 Protein information
  • Uniprot ID ATP7B_MOUSE
  • Uniprot AC Q64446; B1AQ56;
  • UniGene Mm.87854;
  • GeneID 11979
  • KEGG mmu:11979;
  • Mouse Copper-transporting ATPase 2 Protein SEQUENCE
  • SEQUENCE 1462 AA; 157190 MW; BEC219F80EB74214 CRC64;

    MDPRKNLASV GTMPEQERQV TAKEASRKIL SKLALPGRPW EQSMKQSFAF

    DNVGYEGGLD STSSSPAATD VVNILGMTCH SCVKSIEDRI SSLKGIVNIK

    VSLEQGSATV RYVPSVMNLQ QICLQIEDMG FEASAAEGKA ASWPSRSSPA

    QEAVVKLRVE GMTCQSCVSS IEGKIRKLQG VVRIKVSLSN QEAVITYQPY

    LIQPEDLRDH ICDMGFEAAI KNRTAPLRLG PIDVNKLEST NLKKETVSPV

    QISNHFETLG HQGSYLATLP LRIDGMHCKS CVLNIEGNIG QLPGVQNIHV

    SLENKTAQIQ YDPSCVTPMF LQTAIEALPP GHFKVSLPDG VEENEPQSGS

    SQRHQEQGPG RTAVLTISGI TCASSVQPIE DMLSQRKGVQ QTSISLAEGT

    GAVLYDPSIV SLDELRTAVE DMGFEVSVNS ETFTINPVRN FKSGNSVPQT

    MGDIAGSVQK MAPDTRGLPT HQGPGHSSET PSSPGATASQ KCFVQIKGMT

    CASCVSNIER SLQRHAGILS VLVALMSGKA EVKYDPEIIQ SPRIAQLIQD

    LGFEASVMED NTVSEGDIEL IITGMTCASC VHNIESKLTR TNGITYASVA

    LATSKAHVKF DPEIVGPRDI IKIIEEIGFH ASLAQRNPNA HHLDHKTEIK

    QWKKSFLCSL VFGIPVMGLM VYMLIPSSTP QETMVLDHNI IPGLSVLNLI

    FFILCTFVQF LGGWYFYVQA YKSLRHRSAN MDVLIVLATT IAYAYSLVIL

    VVAVAEKAEK SPVTFFDTPP MLFVFIALGR WLEHVAKSKT SEALAKLMSL

    QATEATVVTL GEDNLILREE QVPMELVQRG DVIKVVPGGK FPVDGKVLEG

    NTMADESLIT GEAMPVTKKP GSIVIAGSIN AHGSVLLKAT HVGNDTTLAQ

    IVKLVEEAQM SKAPIQQLAD RFSGYFVPFI IIISTLTLVV WIVIGFVDFG

    VVQKYFPSPS KHISQTEVII RFAFQTSITV LCIACPCSLG LATPTAVMVG

    TGVAAQNGVL IKGGKPLEMA HKIKTVMFDK TGTITHGVPR VMRFLLLADV

    ATLPLRKVLA VVGTAEASSE HPLGVAVTKY CKEELGTETL GYSTDFQAVP

    GCGISCKVSN VEGILARSDL TAHPVGVGNP PTGEGAGPQT FSVLIGNREW

    MRRNGLTISS DISDAMTDHE MKGQTAILVA IDGVLCGMIA IADAVKPEAA

    LAIYTLKSMG VDVALITGDN RKTARAIATQ VGINKVFAEV LPSHKVAKVQ

    ELQNEGKKVA MVGDGVNDSP ALAQADVGIA IGTGTDVAIE AADVVLIRND

    LLDVVASIHL SKRTVRRIRV NLVLALIYNM VGIPIAAGVF MPIGIVLQPW

    MGSAAMAASS VSVVLSSLQL KCYRKPDLER YEAQAHGRMK PLSASQVSVH

    IGMDDRRRDS PRATAWDQVS YVSQVSLSSL TSDRLSRHGG AAEDGGDKWS

    LLLSDRDEEQ CI

  • UCSC uc009lck.1; mouse.;



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