Human BAK1 ELISA Kit

Alternative name
Human apoptosis regulator BAK ELISA Kit;Human bcl-2-like protein 7 ELISA Kit;Human bcl2-L-7 ELISA Kit;Human BAK ELISA Kit;Human BCL2L7 ELISA Kit;Human CDN1 ELISA Kit;Human BAK-LIKE ELISA Kit;Human BCL2 antagonist/killer 1 ELISA Kit;Human bcl-2 homologous antagonist/killer ELISA Kit;Human BCL2-like 7 protein ELISA Kit;Human pro-apoptotic protein BAK ELISA Kit;
Catalog
E004521
species
Human
GeneBAK1
Standard Curve
Other Species Mouse Bak1 ELISA Kit
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity0.1 ng/mL.
Intended UseHuman BAK1 ELISA Kit allows for the in vitro quantitative determination of BAK1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Categories/FamilyCell Biology
Product Description
specificalPrinciple of the Assay: BAK1 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for BAK1. Standards or samples are then added to the microtiter plate wells and BAK1 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of BAK1 present in the sample, a standardized preparation of horseradish peroxidase (HRP) -conjugated polyclonal antibody, specific for BAK1 are added to each well to "sandwich" the BAK1 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain BAK1 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The BAK1 concentration in each sample is interpolated from this standard curve.
Human Bcl-2 homologous antagonist/killer Protein information
Uniprot ID BAK_HUMAN
Uniprot AC Q16611; C0H5Y7; Q6I9T6; Q92533;
UniGene Hs.485139;
GeneID 578
KEGG hsa:578;
Human Bcl-2 homologous antagonist/killer Protein SEQUENCE
SEQUENCE 211 AA; 23409 MW; A2200FE72A46D04E CRC64;
MASGQGPGPP RQECGEPALP SASEEQVAQD TEEVFRSYVF YRHQQEQEAE
GVAAPADPEM VTLPLQPSST MGQVGRQLAI IGDDINRRYD SEFQTMLQHL
QPTAENAYEY FTKIATSLFE SGINWGRVVA LLGFGYRLAL HVYQHGLTGF
LGQVTRFVVD FMLHHCIARW IAQRGGWVAA LNLGNGPILN VLVVLGVVLL
GQFVVRRFFK S
UCSC uc003oes.4; human. [Q16611-1];

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Human BAK1 ELISA Kit