Mouse Aspn ELISA Kit

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  • Alternative name

    Mouse periodontal ligament-associated protein 1 ELISA Kit;Mouse PLAP-1 ELISA Kit;Mouse 4631401G09Rik ELISA Kit;Mouse AA986886 ELISA Kit;Mouse Plap1 ELISA Kit;Mouse Slrr1c ELISA Kit;Mouse asporin ELISA Kit;

  • Catalog
    E044983
  • species
    Mouse
  • GeneAspn
  • Standard CurveMouse Aspn ELISA Kit
  • Other Species Human ASPN ELISA KitBovine ASPN ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of ASPN. No significant cross-reactivity or interference between ASPN and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between ASPN and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity1.0 pg/mL.
  • Intended UseMouse Aspn ELISA Kit allows for the in vitro quantitative determination of Aspn , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Categories/FamilySignal Transduction
  • Product Description
    specifical
    Principle of the assay: ASPN ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for ASPN. Standards or samples are then added to the microtiter plate wells and ASPN if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of ASPN present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for ASPN are added to each well to "sandwich" the ASPN immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain ASPN and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ASPN concentration in each sample is interpolated from this standard curve.
  • Mouse Asporin Protein information
  • Uniprot ID ASPN_MOUSE
  • Uniprot AC Q99MQ4; Q9D6A2;
  • UniGene Mm.383216;
  • GeneID 66695
  • KEGG mmu:66695;
  • Mouse Asporin Protein SEQUENCE
  • SEQUENCE 373 AA; 42573 MW; A50C4C82AABCFC35 CRC64;

    MKEYVMLLLL AVCSAKPFFS PSHTALKNMM LKDMEDTDDD DNDDDDNSLF

    PTKEPVNPFF PFDLFPTCPF GCQCYSRVVH CSDLGLTSVP NNIPFDTRMV

    DLQNNKIKEI KENDFKGLTS LYALILNNNK LTKIHPKTFL TTKKLRRLYL

    SHNQLSEIPL NLPKSLAELR IHDNKVKKIQ KDTFKGMNAL HVLEMSANPL

    ENNGIEPGAF EGVTVFHIRI AEAKLTSIPK GLPPTLLELH LDFNKISTVE

    LEDLKRYREL QRLGLGNNRI TDIENGTFAN IPRVREIHLE HNKLKKIPSG

    LQELKYLQII FLHYNSIAKV GVNDFCPTVP KMKKSLYSAI SLFNNPMKYW

    EIQPATFRCV LGRMSVQLGN VGK

  • UCSC uc007qjm.2; mouse.;


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