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Alternative name
Class A basic helix-loop-helix protein 15 ELISA KIT; Class B basic helix-loop-helix protein 8 ELISA KIT; bHLHb8 ELISA KIT; Muscle, intestine and stomach expression 1 ELISA KIT; MIST-1Bhlha15 ELISA KIT; Bhlhb8 ELISA KIT; Mist1 ELISA KIT; bHLHa15 ELISA KIT; bHLHb8 ELISA KIT; MIST-1 ELISA KIT
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Catalog
E004481
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species
Human
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GeneBHLHB8
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Standard Curve
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Other Species
Mouse BHLHB8 ELISA Kit
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SpecificityThis assay has high sensitivity and excellent specificity for detection of Basic Helix Loop Helix Domain Containing Protein B8 (BHLHB8).
No significant cross-reactivity or interference between Basic Helix Loop Helix Domain Containing Protein B8 (BHLHB8) and analogues was observed.
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SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
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SensitivityTypically less than 0.071ng/mL.
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Intended UseHuman BHLHB8 ELISA Kit allows for the in vitro quantitative determination of BHLHB8 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
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StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
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Product Description
specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Basic Helix Loop Helix Domain Containing Protein B8 (BHLHB8). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Basic Helix Loop Helix Domain Containing Protein B8 (BHLHB8). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Basic Helix Loop Helix Domain Containing Protein B8 (BHLHB8), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Basic Helix Loop Helix Domain Containing Protein B8 (BHLHB8) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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