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Mouse Arg1 ELISA Kit

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  • Alternative name

    Mouse liver-type arginase ELISA Kit;Mouse type I arginase ELISA Kit;Mouse AI ELISA Kit;Mouse AI256583 ELISA Kit;Mouse Arg-1 ELISA Kit;Mouse PGIF ELISA Kit;Mouse arginase, liver ELISA Kit;Mouse arginase-1 ELISA Kit;Mouse arginase 1, liver ELISA Kit;Mouse arginase I ELISA Kit;
  • Catalog
    E044792
  • species
    Mouse
  • GeneArg1
  • Standard CurveMouse Arg1 ELISA Kit
  • Other Species Human ARG1 ELISA KitBovine ARG1 ELISA KitPorcine ARG1 ELISA KitRabbit ARG1 ELISA KitRat Arg1 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of L-ARG1. No significant cross-reactivity or interference between L-ARG1 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between L-ARG1 and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity1.0 ng/mL.
  • Intended UseMouse Arg1 ELISA Kit allows for the in vitro quantitative determination of Arg1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Categories/FamilySignal Transduction
  • Product Description
    specifical    Intended Uses: This L-ARG1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse L-ARG1. This ELISA kit for research use only, not for therapeutic or diagnostic applications! Principle of the Assay||L-ARG1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-L-ARG1 antibody and an L-ARG1-HRP conjugate. The assay sample and buffer are incubated together with L-ARG1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the L-ARG1 concentration since L-ARG1 from samples and L-ARG1-HRP conjugate compete for the anti-L-ARG1 antibody binding site. Since the number of sites is limited, as more sites are occupied by L-ARG1 from the sample, fewer sites are left to bind L-ARG1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The L-ARG1 concentration in each sample is interpolated from this standard curve.
  • Mouse Arginase-1 Protein information
  • Uniprot ID ARGI1_MOUSE
  • Uniprot AC Q61176; Q3TB74; Q3UEL0; Q4FK78; Q80VI4;
  • UniGene Mm.154144;
  • GeneID 11846
  • KEGG mmu:11846;
  • Mouse Arginase-1 Protein SEQUENCE

  • SEQUENCE 323 AA; 34808 MW; 6D0231978AC1B977 CRC64;

    MSSKPKSLEI IGAPFSKGQP RGGVEKGPAA LRKAGLLEKL KETEYDVRDH

    GDLAFVDVPN DSSFQIVKNP RSVGKANEEL AGVVAEVQKN GRVSVVLGGD

    HSLAVGSISG HARVHPDLCV IWVDAHTDIN TPLTTSSGNL HGQPVSFLLK

    ELKGKFPDVP GFSWVTPCIS AKDIVYIGLR DVDPGEHYII KTLGIKYFSM

    TEVDKLGIGK VMEETFSYLL GRKKRPIHLS FDVDGLDPAF TPATGTPVLG

    GLSYREGLYI TEEIYKTGLL SGLDIMEVNP TLGKTAEEVK STVNTAVALT

    LACFGTQREG NHKPGTDYLK PPK

  • UCSC uc007erg.2; mouse.;



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