Mouse Alox15 ELISA Kit

Alternative name
Mouse 15-LOX ELISA Kit;Mouse 12/15-lipoxygenase ELISA Kit;Mouse 12/15-LO ELISA Kit;Mouse arachidonate 12-lipoxygenase, leukocyte-type ELISA Kit;Mouse 12-LOX ELISA Kit;Mouse L-12LO ELISA Kit;Mouse arachidonate omega-6 lipoxygenase ELISA Kit;Mouse Alox12l ELISA Kit;Mouse 12-LO ELISA Kit;Mouse arachidonate 15-lipoxygenase ELISA Kit;
Catalog
E044740
species
Mouse
GeneAlox15
Standard Curve
Other Species Human ALOX15 ELISA Kit Bovine ALOX15 ELISA Kit Porcine ALOX15 ELISA Kit Rabbit ALOX15 ELISA Kit Rat Alox15 ELISA Kit
SpecificityThis assay has high sensitivity and excellent specificity for detection of ALOX15/LOG15. No significant cross-reactivity or interference between ALOX15/LOG15 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between ALOX15/LOG15 and all the analogues, therefore, cross reaction may still exist in some cases.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity0.1 ng/mL.
Intended UseMouse Alox15 ELISA Kit allows for the in vitro quantitative determination of Alox15 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalIntended Uses: This ALOX15/LOG15 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse ALOX15/LOG15. This ELISA kit for research use only, not for therapeutic or diagnostic applications! Principle of the Assay||ALOX15/LOG15 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-ALOX15/LOG15 antibody and an ALOX15/LOG15-HRP conjugate. The assay sample and buffer are incubated together with ALOX15/LOG15-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ALOX15/LOG15 concentration since ALOX15/LOG15 from samples and ALOX15/LOG15-HRP conjugate compete for the anti-ALOX15/LOG15 antibody binding site. Since the number of sites is limited, as more sites are occupied by ALOX15/LOG15 from the sample, fewer sites are left to bind ALOX15/LOG15-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ALOX15/LOG15 concentration in each sample is interpolated from this standard curve.
Mouse Arachidonate 15-lipoxygenase Protein information
Uniprot ID LOX15_MOUSE
Uniprot AC P39654; Q4FJY9; Q5F2E3; Q6PHB2;
UniGene Mm.4584;
GeneID 11687
KEGG mmu:11687;
Mouse Arachidonate 15-lipoxygenase Protein SEQUENCE
SEQUENCE 663 AA; 75445 MW; 0D5412B4502B5799 CRC64;
MGVYRIRVST GDSVYAGSNN EVYLWLIGQH GEASLGKLFR PCRNSEAEFK
VDVSEYLGPL LFVRVQKWHY LKEDAWFCNW ISVKGPGDQG SEYTFPCYRW
VQGTSILNLP EGTGCTVVED SQGLFRNHRE EELEERRSLY RWGNWKDGTI
LNVAATSISD LPVDQRFRED KRLEFEASQV LGTMDTVINF PKNTVTCWKS
LDDFNYVFKS GHTKMAERVR NSWKEDAFFG YQFLNGANPM VLKRSTCLPA
RLVFPPGMEK LQAQLDEELK KGTLFEADFF LLDGIKANVI LCSQQYLAAP
LVMLKLQPDG QLLPIAIQLE LPKTGSTPPP IFTPLDPPMD WLLAKCWVRS
SDLQLHELQA HLLRGHLVAE VFAVATMRCL PSVHPVFKLL VPHLLYTMEI
NVRARSDLIS ERGFFDKVMS TGGGGHLDLL KQAGAFLTYS SLCPPDDLAE
RGLLDIDTCF YAKDALQLWQ VMNRYVVGMF DLYYKTDQAV QDDYELQSWC
QEITEIGLQG AQDRGFPTSL QSRAQACHFI TMCIFTCTAQ HSSIHLGQLD
WFYWVPNAPC TMRLPPPKTK DATMEKLMAT LPNPNQSTLQ INVVWLLGRR
QAVMVPLGQH SEEHFPNPEA KAVLKKFREE LAALDKEIEI RNKSLDIPYE
YLRPSLVENS VAI
UCSC uc007juo.1; mouse.;

Category

Mouse Alox15 ELISA Kit