Mouse Aqp4 ELISA Kit

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  • Alternative name

    Mouse AQP-4 ELISA Kit;Mouse mercurial-insensitive water channel ELISA Kit;Mouse MIWC ELISA Kit;Mouse WCH4 ELISA Kit;Mouse aquaporin 4 ELISA Kit;Mouse aquaporin-4 ELISA Kit;

  • Catalog
    E044710
  • species
    Mouse
  • GeneAqp4
  • Standard CurveMouse Aqp4 ELISA Kit
  • Other Species Human AQP4 ELISA KitHuman AQP4-Ab ELISA KitBovine AQP4 ELISA KitRat Aqp4 ELISA Kit
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.1 ng/mL.
  • Intended UseMouse Aqp4 ELISA Kit allows for the in vitro quantitative determination of Aqp4 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Categories/FamilySignal Transduction
  • Product Description
    specifical
    Principle of the Assay: AQP-4 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-AQP-4 antibody and an AQP-4-HRP conjugate. The assay sample and buffer are incubated together with AQP-4-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the AQP-4 concentration since AQP-4 from samples and AQP-4-HRP conjugate compete for the anti-AQP-4 antibody binding site. Since the number of sites is limited, as more sites are occupied by AQP-4 from the sample, fewer sites are left to bind AQP-4-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The AQP-4 concentration in each sample is interpolated from this standard curve.
  • Mouse Aquaporin-4 Protein information
  • Uniprot ID AQP4_MOUSE
  • Uniprot AC P55088; P97818; Q4FJP1; Q61131; Q61132; Q8VHE4; Q8VHE5; Q9EQI3;
  • UniGene Mm.250786; Mm.489568;
  • GeneID 11829
  • KEGG mmu:11829;
  • Mouse Aquaporin-4 Protein SEQUENCE
  • SEQUENCE 323 AA; 34436 MW; 9AC638A3C3F412E2 CRC64;

    MSDGAAARRW GKCGHSCSRE SIMVAFKGVW TQAFWKAVSA EFLATLIFVL

    LGVGSTINWG GSENPLPVDM VLISLCFGLS IATMVQCFGH ISGGHINPAV

    TVAMVCTRKI SIAKSVFYII AQCLGAIIGA GILYLVTPPS VVGGLGVTTV

    HGNLTAGHGL LVELIITFQL VFTIFASCDS KRTDVTGSIA LAIGFSVAIG

    HLFAINYTGA SMNPARSFGP AVIMGNWANH WIYWVGPIMG AVLAGALYEY

    VFCPDVELKR RLKEAFSKAA QQTKGSYMEV EDNRSQVETE DLILKPGVVH

    VIDIDRGEEK KGKDSSGEVL SSV

  • UCSC uc008eds.2; mouse. [P55088-3];



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