Mouse Aqp4 ELISA Kit

Alternative name
Mouse AQP-4 ELISA Kit;Mouse mercurial-insensitive water channel ELISA Kit;Mouse MIWC ELISA Kit;Mouse WCH4 ELISA Kit;Mouse aquaporin 4 ELISA Kit;Mouse aquaporin-4 ELISA Kit;
Catalog
E044710
species
Mouse
GeneAqp4
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity0.1 ng/mL.
Intended UseMouse Aqp4 ELISA Kit allows for the in vitro quantitative determination of Aqp4 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Categories/FamilySignal Transduction
Product Description
specificalPrinciple of the Assay: AQP-4 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-AQP-4 antibody and an AQP-4-HRP conjugate. The assay sample and buffer are incubated together with AQP-4-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the AQP-4 concentration since AQP-4 from samples and AQP-4-HRP conjugate compete for the anti-AQP-4 antibody binding site. Since the number of sites is limited, as more sites are occupied by AQP-4 from the sample, fewer sites are left to bind AQP-4-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The AQP-4 concentration in each sample is interpolated from this standard curve.
Mouse Aquaporin-4 Protein information
Uniprot ID AQP4_MOUSE
Uniprot AC P55088; P97818; Q4FJP1; Q61131; Q61132; Q8VHE4; Q8VHE5; Q9EQI3;
UniGene Mm.250786; Mm.489568;
GeneID 11829
KEGG mmu:11829;
Mouse Aquaporin-4 Protein SEQUENCE
SEQUENCE 323 AA; 34436 MW; 9AC638A3C3F412E2 CRC64;
MSDGAAARRW GKCGHSCSRE SIMVAFKGVW TQAFWKAVSA EFLATLIFVL
LGVGSTINWG GSENPLPVDM VLISLCFGLS IATMVQCFGH ISGGHINPAV
TVAMVCTRKI SIAKSVFYII AQCLGAIIGA GILYLVTPPS VVGGLGVTTV
HGNLTAGHGL LVELIITFQL VFTIFASCDS KRTDVTGSIA LAIGFSVAIG
HLFAINYTGA SMNPARSFGP AVIMGNWANH WIYWVGPIMG AVLAGALYEY
VFCPDVELKR RLKEAFSKAA QQTKGSYMEV EDNRSQVETE DLILKPGVVH
VIDIDRGEEK KGKDSSGEVL SSV
UCSC uc008eds.2; mouse. [P55088-3];

Category

Mouse Aqp4 ELISA Kit

Mouse AQP-4 ELISA Kit;Mouse mercurial-insensitive water channel ELISA Kit;Mouse MIWC ELISA Kit;Mouse WCH4 ELISA Kit;Mouse aquaporin 4 ELISA Kit;Mouse aquaporin-4 ELISA Kit;