Human BCAM ELISA Kit

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  • Alternative name

    Human Auberger b antigen ELISA Kit;Human B-CAM cell surface glycoprotein ELISA Kit;Human F8/G253 antigen ELISA Kit;Human Lutheran antigen ELISA Kit;Human Lutheran blood group glycoprotein ELISA Kit;Human LU ELISA Kit;Human MSK19 ELISA Kit;Human AU ELISA Kit;Human CD239 ELISA Kit;Human basal cell adhesion molecule (Lutheran blood group) ELISA Kit;Human basal cell adhesion molecule ELISA Kit;Human B-cell adhesion molecule ELISA Kit;Human Lutheran blood group variant LUGA ELISA Kit;Human basal cell adhesion molecule (Lu and Au blood groups) ELISA Kit;

  • Catalog
    E004460
  • species
    Human
  • GeneBCAM
  • Standard CurveHuman BCAM ELISA Kit
  • Other Species Mouse Bcam ELISA KitBovine BCAM ELISA KitRat Bcam ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Basal Cell Adhesion Molecule (BCAM). No significant cross-reactivity or interference between Basal Cell Adhesion Molecule (BCAM) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.60ng/mL.
  • Intended UseHuman BCAM ELISA Kit allows for the in vitro quantitative determination of BCAM , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Basal Cell Adhesion Molecule (BCAM). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Basal Cell Adhesion Molecule (BCAM). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Basal Cell Adhesion Molecule (BCAM), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Basal Cell Adhesion Molecule (BCAM) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Basal cell adhesion molecule Protein information
  • Uniprot ID BCAM_HUMAN
  • Uniprot AC P50895; A8MYF9; A9YWT5; A9YWT6; Q86VC7;
  • UniGene Hs.625725;
  • GeneID 4059
  • KEGG hsa:4059;
  • Human Basal cell adhesion molecule Protein SEQUENCE
  • SEQUENCE 628 AA; 67405 MW; C88F4F5C640C3F5B CRC64;

    MEPPDAPAQA RGAPRLLLLA VLLAAHPDAQ AEVRLSVPPL VEVMRGKSVI

    LDCTPTGTHD HYMLEWFLTD RSGARPRLAS AEMQGSELQV TMHDTRGRSP

    PYQLDSQGRL VLAEAQVGDE RDYVCVVRAG AAGTAEATAR LNVFAKPEAT

    EVSPNKGTLS VMEDSAQEIA TCNSRNGNPA PKITWYRNGQ RLEVPVEMNP

    EGYMTSRTVR EASGLLSLTS TLYLRLRKDD RDASFHCAAH YSLPEGRHGR

    LDSPTFHLTL HYPTEHVQFW VGSPSTPAGW VREGDTVQLL CRGDGSPSPE

    YTLFRLQDEQ EEVLNVNLEG NLTLEGVTRG QSGTYGCRVE DYDAADDVQL

    SKTLELRVAY LDPLELSEGK VLSLPLNSSA VVNCSVHGLP TPALRWTKDS

    TPLGDGPMLS LSSITFDSNG TYVCEASLPT VPVLSRTQNF TLLVQGSPEL

    KTAEIEPKAD GSWREGDEVT LICSARGHPD PKLSWSQLGG SPAEPIPGRQ

    GWVSSSLTLK VTSALSRDGI SCEASNPHGN KRHVFHFGTV SPQTSQAGVA

    VMAVAVSVGL LLLVVAVFYC VRRKGGPCCR QRREKGAPPP GEPGLSHSGS

    EQPEQTGLLM GGASGGARGG SGGFGDEC

  • UCSC uc002ozu.5; human.;




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