Human BAG3 ELISA Kit

Alternative name
Human BAG-3 ELISA Kit;Human Bcl-2-associated athanogene 3 ELISA Kit;Human bcl-2-binding protein Bis ELISA Kit;Human docking protein CAIR-1 ELISA Kit;Human BIS ELISA Kit;Human CAIR-1 ELISA Kit;Human MFM6 ELISA Kit;Human BCL2 associated athanogene 3 ELISA Kit;Human BAG family molecular chaperone regulator 3 ELISA Kit;Human BCL2-binding athanogene 3 ELISA Kit;
Catalog
E004436
species
Human
GeneBAG3
Other Species Mouse Bag3 ELISA Kit
SpecificityThis assay has high sensitivity and excellent specificity for detection of Human BAG3. No significant cross-reactivity or interference between Human BAG3 and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between Human BAG3 and all the analogues, therefore, cross reaction may still exist.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity0.094ng/mL
Intended UseHuman BAG3 ELISA Kit allows for the in vitro quantitative determination of BAG3 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalDescription: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of BAG3 in human serum, plasma and other biological fluids Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human BAG3. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Human BAG3 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human BAG3, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Human BAG3. You can calculate the concentration of Human BAG3 in the samples by comparing the OD of the samples to the standard curve.
Human BAG family molecular chaperone regulator 3 Protein information
Uniprot ID BAG3_HUMAN
Uniprot AC O95817; A8K5L8; Q3B763; Q9NT20; Q9P120;
UniGene Hs.523309;
GeneID 9531
KEGG hsa:9531;
Human BAG family molecular chaperone regulator 3 Protein SEQUENCE
SEQUENCE 575 AA; 61595 MW; A6328A44F37A406A CRC64;
MSAATHSPMM QVASGNGDRD PLPPGWEIKI DPQTGWPFFV DHNSRTTTWN
DPRVPSEGPK ETPSSANGPS REGSRLPPAR EGHPVYPQLR PGYIPIPVLH
EGAENRQVHP FHVYPQPGMQ RFRTEAAAAA PQRSQSPLRG MPETTQPDKQ
CGQVAAAAAA QPPASHGPER SQSPAASDCS SSSSSASLPS SGRSSLGSHQ
LPRGYISIPV IHEQNVTRPA AQPSFHQAQK THYPAQQGEY QTHQPVYHKI
QGDDWEPRPL RAASPFRSSV QGASSREGSP ARSSTPLHSP SPIRVHTVVD
RPQQPMTHRE TAPVSQPENK PESKPGPVGP ELPPGHIPIQ VIRKEVDSKP
VSQKPPPPSE KVEVKVPPAP VPCPPPSPGP SAVPSSPKSV ATEERAAPST
APAEATPPKP GEAEAPPKHP GVLKVEAILE KVQGLEQAVD NFEGKKTDKK
YLMIEEYLTK ELLALDSVDP EGRADVRQAR RDGVRKVQTI LEKLEQKAID
VPGQVQVYEL QPSNLEADQP LQAIMEMGAV AADKGKKNAG NAEDPHTETQ
QPEATAAATS NPSSMTDTPG NPAAP
UCSC uc001lem.4; human.;

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Human BAG3 ELISA Kit