Human TNFSF13B ELISA Kit

Alternative name
Human B lymphocyte stimulator ELISA Kit;Human BLYS ELISA Kit;Human B-cell-activating factor ELISA Kit;Human BAFF ELISA Kit;Human dendritic cell-derived TNF-like molecule ELISA Kit;Human TNF- and APOL-related leukocyte expressed ligand 1 ELISA Kit;Human TALL-1 ELISA Kit;Human TALL1 ELISA Kit;Human TNFSF20 ELISA Kit;Human ZTNF4 ELISA Kit;Human CD257 ELISA Kit;Human DTL ELISA Kit;Human THANK ELISA Kit;Human TNLG7A ELISA Kit;Human tumor necrosis factor superfamily member 13b ELISA Kit;Human tumor necrosis factor ligand superfamily member 13B ELISA Kit;Human ApoL related ligand TALL-1 ELISA Kit;Human B-lymphocyte stimulator ELISA Kit;Human Delta4 BAFF ELISA Kit;Human TNF and ApoL-related leukocyte expressed ligand 1 ELISA Kit;Human TNF homolog that activates apoptosis ELISA Kit;Human delta BAFF ELISA Kit;Human tumor necrosis factor (ligand) superfamily, member 13b ELISA Kit;Human tumor necrosis factor (ligand) superfamily, member 20 ELISA Kit;Human tumor necrosis factor ligand 7A ELISA Kit;Human tumor necrosis factor-like protein ZTNF4 ELISA Kit;
Catalog
E004432
species
Human
GeneTNFSF13B
SpecificityNatural and recombinant human BAFF
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity< 2pg/ml
Intended UseHuman TNFSF13B ELISA Kit allows for the in vitro quantitative determination of TNFSF13B , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the assay: human BAFF ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for BAFF has been precoated onto 96-well plates. Standards(NSO, A134-L285) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for BAFF is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human BAFF amount of sample captured in plate. Background: BAFF was regularly detected by enzyme-linked immunosorbent assay in brain tissue lysates and in normal spinal fluid, and in astrocytes by double fluorescence microscopy. BAFF was localized in astrocytes close to BAFF-R-expressing immune cells. BAFF receptors were strongly expressed in situ in primary central nervous system (CNS) lymphomas.1 The TNF superfamily member B cell-activating factor (BAFF) plays an important role inhumoral immunity and in autoimmune diseases, including RA.Local BAFF gene targeting inhibited proinflammatory cytokine expression, suppressed generation of plasma cells and Th17 cells, and markedly ameliorated joint pathology.2 The B cell activating factor BAFF (BlyS/TALL-1/zTNF4) is a tumor necrosis factor (TNF)-related ligandthat promotes B cell survival and binds to three receptors (BCMA, TACI, and the recently described BAFF-R).3Human BAFF was mapped to chromosome 13q32-34.4 The standard used in this kit is recombinant soluble humanBAFF (A134-L295) with the molecular mass of 19.6KDa.
Human Tumor necrosis factor ligand superfamily member 13b, soluble form Protein information
Uniprot ID TN13B_HUMAN
Uniprot AC Q9Y275; E0ADT7; Q6FHD6; Q7Z5J2;
UniGene Hs.525157;
GeneID 10673
KEGG hsa:10673;
Human Tumor necrosis factor ligand superfamily member 13b, soluble form Protein SEQUENCE
SEQUENCE 285 AA; 31223 MW; 48ED0D7AB38C8867 CRC64;
MDDSTEREQS RLTSCLKKRE EMKLKECVSI LPRKESPSVR SSKDGKLLAA
TLLLALLSCC LTVVSFYQVA ALQGDLASLR AELQGHHAEK LPAGAGAPKA
GLEEAPAVTA GLKIFEPPAP GEGNSSQNSR NKRAVQGPEE TVTQDCLQLI
ADSETPTIQK GSYTFVPWLL SFKRGSALEE KENKILVKET GYFFIYGQVL
YTDKTYAMGH LIQRKKVHVF GDELSLVTLF RCIQNMPETL PNNSCYSAGI
AKLEEGDELQ LAIPRENAQI SLDGDVTFFG ALKLL
UCSC uc001vqr.4; human. [Q9Y275-1];

Human TNFSF13B ELISA Kit

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