Mouse Mbp ELISA Kit

Alternative name
Mouse MBP ELISA Kit;Mouse myelin A1 protein ELISA Kit;Mouse shi ELISA Kit;Mouse C76307 ELISA Kit;Mouse Hmbpr ELISA Kit;Mouse R75289 ELISA Kit;Mouse Golli-Mbp ELISA Kit;Mouse jve ELISA Kit;Mouse mld ELISA Kit;Mouse myelin basic protein ELISA Kit;Mouse myelin deficient ELISA Kit;Mouse shiverer ELISA Kit;
Catalog
E044292
species
Mouse
GeneMbp
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Intended UseMouse Mbp ELISA Kit allows for the in vitro quantitative determination of Mbp , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Categories/FamilyCell Biology
Product Description
specificalFor Samples: Cell culture fluid & body fluid & tissue homogenate Serum or blood plasma Intended Uses: This MBP-AB ELISA kit is intended for laboratory research use only and not for use in diagnostic or therapeutic procedures. The stop solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration of MBP-AB in the sample, this MBP-AB ELISA Kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versus MBP-AB concentration. The concentration of in the samples is then determined by comparing the O.D. of the samples to the standard curve.fg Principle of the Assay: The coated well immunoenzymatic assay for the quantitative measurement of MBP-AB utilizes a multiclonal anti-MBP-AB antibody and an MBP-AB-HRP conjugate. The assay sample and buffer are incubated together with MBP-AB-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the MBP-AB concentration since MBP-AB from samples and MBP-AB-HRP conjugate compete for the anti-MBP-AB antibody binding site. Since the number of sites is limited, as more sites are occupied by MBP-AB from the sample, fewer sites are left to bind MBP-AB-HRP conjugate. Standards of known MBP-AB concentrations are run concurrently with the samples being assayed and a standard curve is plotted relating the intensity of the color (Optical Density) to the concentration of MBP-AB. The MBP-AB concentration in each sample is interpolated from this standard curve.
Mouse Myelin basic protein Protein information
Uniprot ID MBP_MOUSE
Uniprot AC P04370; Q01585; Q03139; Q03176; Q61836; Q61837; Q99KE4; Q9QWP1;
UniGene Mm.252063; Mm.454459;
GeneID 17196
KEGG mmu:17196;
Mouse Myelin basic protein Protein SEQUENCE
SEQUENCE 250 AA; 27168 MW; B418ED11C27B0C43 CRC64;
MGNHSGKREL SAEKASKDGE IHRGEAGKKR SVGKLSQTAS EDSDVFGEAD
AIQNNGTSAE DTAVTDSKHT ADPKNNWQGA HPADPGNRPH LIRLFSRDAP
GREDNTFKDR PSESDELQTI QEDPTAASGG LDVMASQKRP SQRSKYLATA
STMDHARHGF LPRHRDTGIL DSIGRFFSGD RGAPKRGSGK DSHTRTTHYG
SLPQKSQHGR TQDENPVVHF FKNIVTPRTP PPSQGKGGRD SRSGSPMARR
UCSC uc029tqh.1; mouse. [P04370-14];

Mouse Mbp ELISA Kit

Contact Us