Mouse GM2 Ab IgM ELISA Kit

Contact Us
  • Catalog
  • species
  • GeneGM2 Ab IgM
  • Standard CurveMouse GM2 Ab IgM ELISA Kit
  • Other Species Human GM2 Ab IgM ELISA Kit
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity1.0 ng/mL.
  • Intended UseMouse GM2 Ab IgM ELISA Kit allows for the in vitro quantitative determination of GM2 Ab IgM , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageFor 5-7days:Store the whole kit at 4℃
    For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
  • Product Description
    Intended Uses: This Anti-GM2 (ganglioside) Antibody IgM ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse Anti-GM2 (ganglioside) Antibody IgM. This ELISA kit for research use only, not for therapeutic or diagnostic applications! Principle of the Assay: Anti-GM2 (ganglioside) Antibody IgM ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-Anti-GM2 (ganglioside) Antibody IgM antibody and an Anti-GM2 (ganglioside) Antibody IgM-HRP conjugate. The assay sample and buffer are incubated together with Anti-GM2 (ganglioside) Antibody IgM-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the Anti-GM2 (ganglioside) Antibody IgM concentration since Anti-GM2 (ganglioside) Antibody IgM from samples and Anti-GM2 (ganglioside) Antibody IgM-HRP conjugate compete for the anti-Anti-GM2 (ganglioside) Antibody IgM antibody binding site. Since the number of sites is limited, as more sites are occupied by Anti-GM2 (ganglioside) Antibody IgM from the sample, fewer sites are left to bind Anti-GM2 (ganglioside) Antibody IgM-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The Anti-GM2 (ganglioside) Antibody IgM concentration in each sample is interpolated from this standard curve.

Inquiry About Mouse GM2 Ab IgM ELISA Kit If you hope to order it or contact us directly, please contact us via We will get back to you in 12hrs,Thanks!