SpecificityThis assay has high sensitivity and excellent specificity for detection of GM1Ab. No significant cross-reactivity or interference between GM1Ab and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between GM1Ab and all the analogues, therefore, cross reaction may still exist in some cases.
Intended UseMouse GM1 ELISA Kit allows for the in vitro quantitative determination of GM1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
Product Categories/FamilyCell Biology
Product Description specificalIntended Uses: This GM1Ab ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse GM1Ab. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
Principle of the Assay||GM1Ab ELISA kit applies the competitive enzyme immunoassay technique utilizing Ganglioside M1 antigen and an GM1Ab-HRP conjugate. The assay sample and buffer are incubated together with GM1Ab-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the GM1Ab concentration since GM1Ab from samples and GM1Ab-HRP conjugate compete for the Ganglioside M1 antigen binding site. Since the number of sites is limited, as more sites are occupied by GM1Ab from the sample, fewer sites are left to bind GM1Ab-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The GM1Ab concentration in each sample is interpolated from this standard curve.