Mouse GD1a ELISA Kit

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  • Alternative name

    Histidinol-phosphate aminotransferase 2, chloroplastic ELISA KIT; Gene duplicate 1-A protein ELISA KIT; Imidazole acetol-phosphate transaminase ELISA KIT; Protein HISTIDINE BIOSYNTHESIS 6BHISN6B ELISA KIT; GD1-A ELISA KIT; HPA2 ELISA KIT; At1g71920 ELISA KIT; F17M19.7 ELISA KIT

  • Catalog
    E044205
  • species
    Mouse
  • GeneGD1a
  • Standard CurveMouse GD1a ELISA Kit
  • Other Species Human GD1a ELISA KitHuman GD1a GaINAc ELISA KitMouse GD1a-GaINAc ELISA Kit
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Intended UseMouse GD1a ELISA Kit allows for the in vitro quantitative determination of GD1a , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageFor 5-7days:Store the whole kit at 4℃
    For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
  • Product Categories/FamilyCell Biology
  • Product Description
    specifical
    Principle of the Assay: GD1a-Ab ELISA kit applies the competitive enzyme immunoassay technique utilizing GD1a antigen and an GD1a-Ab-HRP conjugate. The assay sample and buffer are incubated together with GD1a-Ab-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the GD1a-Ab concentration since GD1a-Ab from samples and GD1a-Ab-HRP conjugate compete for the GD1a antigen binding site. Since the number of sites is limited, as more sites are occupied by GD1a-Ab from the sample, fewer sites are left to bind GD1a-Ab-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The GD1a-Ab concentration in each sample is interpolated from this standard curve.


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