Mouse FSH-Ab ELISA Kit

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  • Catalog
  • species
  • Standard CurveMouse FSH-Ab ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of LHAb. No significant cross-reactivity or interference between LHAb and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between LHAb and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity1.0 ng/mL.
  • Intended UseMouse FSH-Ab ELISA Kit allows for the in vitro quantitative determination of , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageFor 5-7days:Store the whole kit at 4℃
    For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
  • Product Categories/FamilySignal Transduction
  • Product Description
    Principle of the assay: LHAb ELISA kit applies the competitive enzyme immunoassay technique utilizing Luteinizing Hormone antigen and an LHAb-HRP conjugate. The assay sample and buffer are incubated together with LHAb-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the LHAb concentration since LHAb from samples and LHAb-HRP conjugate compete for the Luteinizing Hormone antigen binding site. Since the number of sites is limited, as more sites are occupied by LHAb from the sample, fewer sites are left to bind LHAb-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The LHAb concentration in each sample is interpolated from this standard curve.

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