Human BPI ELISA Kit

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  • Alternative name

    Human BPI ELISA Kit;Human CAP 57 ELISA Kit;Human BPIFD1 ELISA Kit;Human rBPI ELISA Kit;Human bactericidal/permeability-increasing protein ELISA Kit;Human bactericidal permeability-increasing protein ELISA Kit;Human BPI fold containing family D, member 1 ELISA Kit;Human recombinant BPI holoprotein, rBPI ELISA Kit;

  • Catalog
    E004408
  • species
    Human
  • GeneBPI
  • Standard CurveHuman BPI ELISA Kit
  • Other Species Human Bpi Ab ELISA KitHuman BPI-Ab ELISA KitMouse Bpi-Ab ELISA KitMouse Bpi ELISA KitBovine BPI ELISA KitRabbit BPI ELISA KitRat Bpi ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Bactericidal/Permeability Increasing Protein (BPI). No significant cross-reactivity or interference between Bactericidal/Permeability Increasing Protein (BPI) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 5.3pg/mL.
  • Intended UseHuman BPI ELISA Kit allows for the in vitro quantitative determination of BPI , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Bactericidal/Permeability Increasing Protein (BPI). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Bactericidal/Permeability Increasing Protein (BPI). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Bactericidal/Permeability Increasing Protein (BPI), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Bactericidal/Permeability Increasing Protein (BPI) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Bactericidal permeability-increasing protein Protein information
  • Uniprot ID BPI_HUMAN
  • Uniprot AC Q9H203; Q9UCT4; Q9UD65;
  • UniGene Hs.529019;
  • GeneID 671
  • KEGG hsa:671;
  • Human Bactericidal permeability-increasing protein Protein SEQUENCE
  • SEQUENCE 487 AA; 53900 MW; 30BC73B1B465B62D CRC64;

    MRENMARGPC NAPRWASLMV LVAIGTAVTA AVNPGVVVRI SQKGLDYASQ

    QGTAALQKEL KRIKIPDYSD SFKIKHLGKG HYSFYSMDIR EFQLPSSQIS

    MVPNVGLKFS ISNANIKISG KWKAQKRFLK MSGNFDLSIE GMSISADLKL

    GSNPTSGKPT ITCSSCSSHI NSVHVHISKS KVGWLIQLFH KKIESALRNK

    MNSQVCEKVT NSVSSELQPY FQTLPVMTKI DSVAGINYGL VAPPATTAET

    LDVQMKGEFY SENHHNPPPF APPVMEFPAA HDRMVYLGLS DYFFNTAGLV

    YQEAGVLKMT LRDDMIPKES KFRLTTKFFG TFLPEVAKKF PNMKIQIHVS

    ASTPPHLSVQ PTGLTFYPAV DVQAFAVLPN SSLASLFLIG MHTTGSMEVS

    AESNRLVGEL KLDRLLLELK HSNIGPFPVE LLQDIMNYIV PILVLPRVNE

    KLQKGFPLPT PARVQLYNVV LQPHQNFLLF GADVVYK

  • UCSC uc002xib.3; human.;




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