Intended UseMouse ANN-II ELISA Kit allows for the in vitro quantitative determination of ANN-II , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
Product Description specificalBackground/Introduction: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated ANN-II concentrations in Mouse serum, plasma and other body fluids. Using Purified Mouse ANN-II antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add ANN-II and ANN-II antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of ANN-II in the samples is then determined by comparing the O.D. of the samples to the standard curve.