Human B7.H3 ELISA Kit

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  • Alternative name

    CD276 antigen ELISA KIT; 4Ig-B7-H3 ELISA KIT; B7 homolog 3 ELISA KIT; B7-H3 ELISA KIT; Costimulatory molecule ELISA KIT; CD_antigen: CD276CD276 ELISA KIT; B7H3 ELISA KIT; PSEC0249 ELISA KIT; UNQ309/PRO352 ELISA KIT; B7-H3 ELISA KIT

  • Catalog
  • species
  • GeneB7.H3
  • Standard CurveHuman B7.H3 ELISA Kit
  • Other Species Human B7-H3 ELISA KitMouse B7-H3 ELISA KitMouse B7.H3 ELISA Kit
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.1 ng/mL.
  • Intended UseHuman B7.H3 ELISA Kit allows for the in vitro quantitative determination of B7.H3 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageFor 5-7days:Store the whole kit at 4℃
    For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
  • Product Categories/FamilyImmunology
  • Product Description
    Principle of the Assay: B7.H3 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for B7.H3. Standards or samples are then added to the microtiter plate wells and B7.H3 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of B7.H3 present in the sample, a standardized preparation of horseradish peroxidase (HRP) -conjugated polyclonal antibody, specific for B7.H3 are added to each well to "sandwich" the B7.H3 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain B7.H3 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The B7.H3 concentration in each sample is interpolated from this standard curve.

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