Human CD80 ELISA Kit

Alternative name
Human activation B7-1 antigen ELISA Kit;Human BB1 ELISA Kit;Human CTLA-4 counter-receptor B7.1 ELISA Kit;Human B7 ELISA Kit;Human CD28LG ELISA Kit;Human CD28LG1 ELISA Kit;Human LAB7 ELISA Kit;Human B7-1 ELISA Kit;Human B7.1 ELISA Kit;Human CD80 molecule ELISA Kit;Human T-lymphocyte activation antigen CD80 ELISA Kit;Human B-lymphocyte activation antigen B7 ELISA Kit;Human CD80 antigen (CD28 antigen ligand 1, B7-1 antigen) ELISA Kit;Human costimulatory factor CD80 ELISA Kit;Human costimulatory molecule variant IgV-CD80 ELISA Kit;
Catalog
E004395
species
Human
GeneCD80
SpecificityNatural and recombinant human CD80
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity< 10pg/ml
Intended UseHuman CD80 ELISA Kit allows for the in vitro quantitative determination of CD80 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the assay: human CD80 ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for CD80 has been precoated onto 96-well plates. Standards(NSO, V35-N242) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CD80 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB are used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human CD80 amount of sample captured in plate. Background: The protein CD80 (Cluster of Differentiation 80) is a molecule found on activated B cells and monocytes which provides a costimulatory signal necessary for T cell activation and survival. It is also known as B7-1. The cDNA for B7-1 predicts a type I membrane protein, i.e., one synthesized with a signal peptide that is cleaved upon translocation across the endoplasmic membrane. The protein is predicted to contain 2 extracellular domains structurally similar to those of Ig, a hydrophobic transmembrane region, and a short cytoplasmic domain.The CD80 and CD86 genes encode B7-1 and B7-2, respectively, which are structurally similar members of the immunoglobulin superfamily expressed on a variety of hematopoietic cell types. B7-1 and B7-2 provide a costimulatory signal to T cells by interacting with CD28 and CTLA4.
Human T-lymphocyte activation antigen CD80 Protein information
Uniprot ID CD80_HUMAN
Uniprot AC P33681; Q5DTA9; Q5DTB0;
UniGene Hs.838;
GeneID 941
KEGG hsa:941;
Human T-lymphocyte activation antigen CD80 Protein SEQUENCE
SEQUENCE 288 AA; 33048 MW; BA453EE34528B1F4 CRC64;
MGHTRRQGTS PSKCPYLNFF QLLVLAGLSH FCSGVIHVTK EVKEVATLSC
GHNVSVEELA QTRIYWQKEK KMVLTMMSGD MNIWPEYKNR TIFDITNNLS
IVILALRPSD EGTYECVVLK YEKDAFKREH LAEVTLSVKA DFPTPSISDF
EIPTSNIRRI ICSTSGGFPE PHLSWLENGE ELNAINTTVS QDPETELYAV
SSKLDFNMTT NHSFMCLIKY GHLRVNQTFN WNTTKQEHFP DNLLPSWAIT
LISVNGIFVI CCLTYCFAPR CRERRRNERL RRESVRPV
UCSC uc003ecq.4; human. [P33681-1];

Human CD80 ELISA Kit

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