Human BRAF ELISA Kit

Alternative name
Human proto-oncogene B-Raf ELISA Kit;Human p94 ELISA Kit;Human v-raf murine sarcoma viral oncogene homolog B1 ELISA Kit;Human BRAF1 ELISA Kit;Human RAFB1 ELISA Kit;Human B-RAF1 ELISA Kit;Human B-raf ELISA Kit;Human NS7 ELISA Kit;Human B-Raf proto-oncogene, serine/threonine kinase ELISA Kit;Human serine/threonine-protein kinase B-raf ELISA Kit;Human 94 kDa B-raf protein ELISA Kit;Human B-Raf proto-oncogene serine/threonine-protein kinase (p94) ELISA Kit;Human murine sarcoma viral (v-raf) oncogene homolog B1 ELISA Kit;Human v-raf murine sarcoma viral oncogene homolog B ELISA Kit;
Catalog
E004389
species
Human
GeneBRAF
Standard Curve
Other Species Mouse Braf ELISA Kit Chicken BRAF ELISA Kit
SpecificityThis assay has high sensitivity and excellent specificity for detection of B-Raf Proto Oncogene Serine/Threonine Protein Kinase (BRAF). No significant cross-reactivity or interference between B-Raf Proto Oncogene Serine/Threonine Protein Kinase (BRAF) and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityTypically less than 0.115ng/mL.
Intended UseHuman BRAF ELISA Kit allows for the in vitro quantitative determination of BRAF , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to B-Raf Proto Oncogene Serine/Threonine Protein Kinase (BRAF). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to B-Raf Proto Oncogene Serine/Threonine Protein Kinase (BRAF). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain B-Raf Proto Oncogene Serine/Threonine Protein Kinase (BRAF), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of B-Raf Proto Oncogene Serine/Threonine Protein Kinase (BRAF) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human Serine/threonine-protein kinase B-raf Protein information
Uniprot ID BRAF_HUMAN
Uniprot AC Q13878; Q3MIN6; Q9UDP8; Q9Y6T3;
UniGene Hs.550061; Hs.600998; Hs.605380; Hs.659507; Hs.684552;
GeneID 673
KEGG hsa:673;
Human Serine/threonine-protein kinase B-raf Protein SEQUENCE
SEQUENCE 766 AA; 84437 MW; 0798C2AAB487E813 CRC64;
MAALSGGGGG GAEPGQALFN GDMEPEAGAG AGAAASSAAD PAIPEEVWNI
KQMIKLTQEH IEALLDKFGG EHNPPSIYLE AYEEYTSKLD ALQQREQQLL
ESLGNGTDFS VSSSASMDTV TSSSSSSLSV LPSSLSVFQN PTDVARSNPK
SPQKPIVRVF LPNKQRTVVP ARCGVTVRDS LKKALMMRGL IPECCAVYRI
QDGEKKPIGW DTDISWLTGE ELHVEVLENV PLTTHNFVRK TFFTLAFCDF
CRKLLFQGFR CQTCGYKFHQ RCSTEVPLMC VNYDQLDLLF VSKFFEHHPI
PQEEASLAET ALTSGSSPSA PASDSIGPQI LTSPSPSKSI PIPQPFRPAD
EDHRNQFGQR DRSSSAPNVH INTIEPVNID DLIRDQGFRG DGGSTTGLSA
TPPASLPGSL TNVKALQKSP GPQRERKSSS SSEDRNRMKT LGRRDSSDDW
EIPDGQITVG QRIGSGSFGT VYKGKWHGDV AVKMLNVTAP TPQQLQAFKN
EVGVLRKTRH VNILLFMGYS TKPQLAIVTQ WCEGSSLYHH LHIIETKFEM
IKLIDIARQT AQGMDYLHAK SIIHRDLKSN NIFLHEDLTV KIGDFGLATV
KSRWSGSHQF EQLSGSILWM APEVIRMQDK NPYSFQSDVY AFGIVLYELM
TGQLPYSNIN NRDQIIFMVG RGYLSPDLSK VRSNCPKAMK RLMAECLKKK
RDERPLFPQI LASIELLARS LPKIHRSASE PSLNRAGFQT EDFSLYACAS
PKTPIQAGGY GAFPVH
UCSC uc003vwc.5; human.;

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Human BRAF ELISA Kit