Mouse AT2R1 ELISA Kit

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  • Alternative name

    Type-1 angiotensin II receptor ELISA KIT; AT1AR ELISA KIT; AT1BR ELISA KIT; Angiotensin II type-1 receptor ELISA KIT; AT1AGTR1 ELISA KIT; AGTR1A ELISA KIT; AGTR1B ELISA KIT; AT2R1 ELISA KIT; AT2R1B ELISA KIT; AT1 ELISA KIT

  • Catalog
    E043851
  • species
    Mouse
  • GeneAT2R1
  • Standard CurveMouse AT2R1 ELISA Kit
  • Other Species Human AT2R1 ELISA KitHuman AT2R1-AA ELISA Kit
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity1.0 ng/mL.
  • Intended UseMouse AT2R1 ELISA Kit allows for the in vitro quantitative determination of AT2R1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageFor 5-7days:Store the whole kit at 4℃
    For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
  • Product Categories/FamilySignal Transduction
  • Product Description
    specifical
    Principle of the Assay: Anti-AT2R1 ELISA kit applies the competitive enzyme immunoassay technique utilizing Angiotensin 2 Receptor 1 Antigen (Anti-AT2R1) and an Anti-AT2R1-HRP conjugate. The assay sample and buffer are incubated together with Anti-AT2R1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the Anti-AT2R1 concentration since Anti-AT2R1 from samples and Anti-AT2R1-HRP conjugate compete for the Angiotensin 2 Receptor 1 Antigen binding site. Since the number of sites is limited, as more sites are occupied by Anti-AT2R1 from the sample, fewer sites are left to bind Anti-AT2R1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The Anti-AT2R1 concentration in each sample is interpolated from this standard curve.


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