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Alternative name
T-lymphocyte activation antigen CD80 ELISA KIT; Activation B7-1 antigen ELISA KIT; B7 ELISA KIT; CD_antigen: CD80Cd80 ELISA KIT; B7 ELISA KIT; B7 ELISA KIT
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Catalog
E004378
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species
Human
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GeneLAB7-1
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Standard Curve
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SpecificityThis assay has high sensitivity and excellent specificity for detection of LAB7-1. No significant cross-reactivity or interference between LAB7-1 and analogues was observed.
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SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
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Sensitivity33pg/mL.
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Intended UseHuman LAB7-1 ELISA Kit allows for the in vitro quantitative determination of LAB7-1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
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StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
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Product Description
specificalIntended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of LAB7-1 in human serum, plasma, tissue homogenates and other biological fluids.
Principle of the Assay||The microtiter plate provided in this kit has been pre-coated with an antibody specific to LAB7-1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to LAB7-1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain LAB7-1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of LAB7-1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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