Mouse Angpt1 ELISA Kit

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  • Alternative name

    Mouse Ang-1 ELISA Kit;Mouse Agpt ELISA Kit;Mouse 1110046O21Rik ELISA Kit;Mouse Ang1 ELISA Kit;Mouse angiopoietin 1 ELISA Kit;Mouse angiopoietin-1 ELISA Kit;

  • Catalog
    E043779
  • species
    Mouse
  • GeneAngpt1
  • Standard CurveMouse Angpt1 ELISA Kit
  • Other Species Human ANGPT1 ELISA KitBovine ANGPT1 ELISA KitCanine ANGPT1 ELISA KitPorcine ANGPT1 ELISA KitRat Angpt1 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Mouse ANGPT1. No significant cross-reactivity or interference between Mouse ANGPT1 and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.078 pg/ml
  • Intended UseMouse Angpt1 ELISA Kit allows for the in vitro quantitative determination of Angpt1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Introduction: The angiopoietins are protein growth factors that promote angiogenesis, the formation of blood vessels from pre-existing blood vessels. All angiopoietins bind with similar affinity to an endothelial cell-specific tyrosine-protein kinase receptor. The mechanism by which they contribute to angiogenesis is thought to involve regulation of endothelial cell interactions with supporting perivascular cells. There are now four identified angiopoietins: Ang-1, Ang-2, Ang-3, Ang-4. Ang-1 is an angiogenic secreted protein that interact with endothelial specific Tie-2 receptor. Mouse and human Ang-1 have 98% identity. During embryonic development, Ang-1 binds and induces tyrosine phosphorylation of Tie-2. Ang-1 deficient mice mimic the phenotype exhibited by animals deficient in Tie-2. The role of Ang-1 in angiogenesis appears to be different from VEGF. Ang-1 is prominently expressed in the myocardium of atrium and ventricle, mesenchymal and smooth muscle cells surrounding most blood vessels, and lung. Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to ANG-1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for ANG-1 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain ANG-1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of ANG-1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Mouse Angiopoietin-1 Protein information
  • Uniprot ID ANGP1_MOUSE
  • Uniprot AC O08538; Q6NWV7;
  • UniGene Mm.309336;
  • GeneID 11600
  • KEGG mmu:11600;
  • Mouse Angiopoietin-1 Protein SEQUENCE
  • SEQUENCE 498 AA; 57519 MW; FC36F905A9E79074 CRC64;

    MTVFLSFAFF AAILTHIGCS NQRRNPENGG RRYNRIQHGQ CAYTFILPEH

    DGNCRESATE QYNTNALQRD APHVEPDFSS QKLQHLEHVM ENYTQWLQKL

    ENYIVENMKS EMAQIQQNAV QNHTATMLEI GTSLLSQTAE QTRKLTDVET

    QVLNQTSRLE IQLLENSLST YKLEKQLLQQ TNEILKIHEK NSLLEHKILE

    MEGKHKEELD TLKEEKENLQ GLVSRQTFII QELEKQLSRA TNNNSILQKQ

    QLELMDTVHN LISLCTKEGV LLKGGKREEE KPFRDCADVY QAGFNKSGIY

    TIYFNNMPEP KKVFCNMDVN GGGWTVIQHR EDGSLDFQRG WKEYKMGFGN

    PSGEYWLGNE FIFAITSQRQ YMLRIELMDW EGNRAYSQYD RFHIGNEKQN

    YRLYLKGHTG TAGKQSSLIL HGADFSTKDA DNDNCMCKCA LMLTGGWWFD

    ACGPSNLNGM FYTAGQNHGK LNGIKWHYFK GPSYSLRSTT MMIRPLDF

  • UCSC uc007vpc.2; mouse.;


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