Mouse Ang ELISA Kit

Alternative name
Mouse angiogenin-1 ELISA Kit;Mouse ribonuclease 5 ELISA Kit;Mouse RNase 5 ELISA Kit;Mouse Ang1 ELISA Kit;Mouse Rnase5 ELISA Kit;Mouse Rnase5a ELISA Kit;Mouse AI385586 ELISA Kit;Mouse angiogenin, ribonuclease, RNase A family, 5 ELISA Kit;Mouse angiogenin ELISA Kit;Mouse angiogenin, ribonuclease A family, member 1 ELISA Kit;
Catalog
E043752
species
Mouse
GeneAng
SpecificityThis assay has high sensitivity and excellent specificity for detection of Mouse ANG. No significant cross-reactivity or interference between Mouse ANG and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between Mouse ANG and all the analogues, therefore, cross reaction may still exist.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity28.125pg/mL
Intended UseMouse Ang ELISA Kit allows for the in vitro quantitative determination of Ang , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalDescription: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of ANG in Mouse serum, plasma and other biological fluids Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse ANG. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Mouse ANG and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse ANG, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Mouse ANG. You can calculate the concentration of Mouse ANG in the samples by comparing the OD of the samples to the standard curve.
Mouse Angiogenin Protein information
Uniprot ID ANGI_MOUSE
Uniprot AC P21570;
UniGene Mm.202665;
GeneID 11727
KEGG mmu:11727;
Mouse Angiogenin Protein SEQUENCE
SEQUENCE 145 AA; 16228 MW; 06944260BB764938 CRC64;
MAISPGPLFL IFVLGLVVIP PTLAQDDSRY TKFLTQHHDA KPKGRDDRYC
ERMMKRRSLT SPCKDVNTFI HGNKSNIKAI CGANGSPYRE NLRMSKSPFQ
VTTCKHTGGS PRPPCQYRAS AGFRHVVIAC ENGLPVHFDE SFFSL
UCSC uc007tml.2; mouse.;

Mouse Ang ELISA Kit

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