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Alternative name
Amyloid beta A4 protein ELISA KIT; ABPP ELISA KIT; APPI ELISA KIT; APP ELISA KIT; Alzheimer disease amyloid protein ELISA KIT; Cerebral vascular amyloid peptide ELISA KIT; CVAP ELISA KIT; PreA4 ELISA KIT; Protease nexin-II ELISA KIT; PN-IIAPP ELISA KIT; A4 ELISA KIT; AD1 ELISA KIT; APP ELISA KIT; CVAP ELISA KIT; PN-II ELISA KIT; S-APP-alpha ELISA KIT; S-APP-beta ELISA KIT; AICD-59 ELISA KIT; AID(59) ELISA KIT; AICD-57 ELISA KIT; AID(57) ELISA KIT; AICD-50 ELISA KIT; AID(50) ELISA KIT
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Catalog
E043695
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species
Mouse
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GeneAbeta40
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Standard Curve
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Other Species
Human Abeta40 ELISA Kit
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SpecificityThis assay has high sensitivity and excellent specificity for detection of Abeta40. No significant cross-reactivity or interference between Abeta40 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between Abeta40 and all the analogues, therefore, cross reaction may still exist in some cases.
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SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
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Sensitivity1.0 pg/mL.
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Intended UseMouse Abeta40 ELISA Kit allows for the in vitro quantitative determination of Abeta40 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
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StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
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Product Categories/FamilyNeurobiology
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Product Description
specificalPrinciple of the Assay: Abeta40 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-Abeta40 antibody and an Abeta40-HRP conjugate. The assay sample and buffer are incubated together with Abeta40-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the Abeta40 concentration since Abeta40 from samples and Abeta40-HRP conjugate compete for the anti-Abeta40 antibody binding site. Since the number of sites is limited, as more sites are occupied by Abeta40 from the sample, fewer sites are left to bind Abeta40-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The Abeta40 concentration in each sample is interpolated from this standard curve.
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