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Alternative name
POTE ankyrin domain family member H ELISA KIT; ANKRD26-like family C member 3 ELISA KIT; Prostate, ovary, testis-expressed protein on chromosome 22 ELISA KIT; POTE-22POTEH ELISA KIT; A26C3 ELISA KIT; ACTBL1 ELISA KIT; POTE22 ELISA KIT; POTE-22 ELISA KIT
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Catalog
E000436
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species
Human
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GeneACTBL1
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SpecificityThis assay has high sensitivity and excellent specificity for detection of human ACTBL1. No significant cross-reactivity or interference between human ACTBL1 and analogues was observed.
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SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
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SensitivityThe minimum detectable dose of human ACTBL1 is typically less than 5.8 pg/ml.The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mea
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Intended UseHuman ACTBL1 ELISA Kit allows for the in vitro quantitative determination of ACTBL1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
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StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
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Product Description
specificalPrinciple of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for ACTBL1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ACTBL1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ACTBL1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ACTBL1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
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