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Alternative name
Alpha-2-macroglobulin ELISA KIT; C3 and PZP-like alpha-2-macroglobulin domain-containing protein 5A2M ELISA KIT; CPAMD5 ELISA KIT; FWP007 ELISA KIT; Alpha-2-M ELISA KIT
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Catalog
E043536
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species
Mouse
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GenePre-alpha2 MG
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Other Species
Human PRE-alpha2 MG ELISA Kit
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SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
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Sensitivity1.0 umol/L.
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Intended UseMouse Pre-alpha2 MG ELISA Kit allows for the in vitro quantitative determination of Pre-alpha2 MG , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
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StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
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Product Categories/FamilySignal Transduction
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Product Description
specificalPrinciple of the Assay: PRE-alpha2 MG ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-PRE-alpha2 MG antibody and an PRE-alpha2 MG-HRP conjugate. The assay sample and buffer are incubated together with PRE-alpha2 MG-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the PRE-alpha2 MG concentration since PRE-alpha2 MG from samples and PRE-alpha2 MG-HRP conjugate compete for the anti-PRE-alpha2 MG antibody binding site. Since the number of sites is limited, as more sites are occupied by PRE-alpha2 MG from the sample, fewer sites are left to bind PRE-alpha2 MG-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PRE-alpha2 MG concentration in each sample is interpolated from this standard curve.
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