Intended UseMouse alpha-TG ELISA Kit allows for the in vitro quantitative determination of alpha-TG , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
Product Description specificalFor samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
INTENDED USE This TRACP5b ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse TRACP5b. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
PRINCIPLE OF THE ASSAY TRACP5b ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-TRACP5b antibody and an TRACP5b-HRP conjugate. The assay sample and buffer are incubated together with TRACP5b-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the TRACP5b concentration since TRACP5b from samples and TRACP5b-HRP conjugate compete for the anti-TRACP5b antibody binding site. Since the number of sites is limited, as more sites are occupied by TRACP5b from the sample, fewer sites are left to bind TRACP5b-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The TRACP5b concentration in each sample is interpolated from this standard curve.