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Alternative name
P-selectin ELISA KIT; CD62 antigen-like family member P ELISA KIT; Granule membrane protein 140 ELISA KIT; GMP-140 ELISA KIT; Leukocyte-endothelial cell adhesion molecule 3 ELISA KIT; LECAM3 ELISA KIT; Platelet activation dependent granule-external membrane protein ELISA KIT; PADGEM ELISA KIT; CD_antigen: CD62PSELP ELISA KIT; GMRP ELISA KIT; GRMP ELISA KIT; GMP-140 ELISA KIT; LECAM3 ELISA KIT; PADGEM ELISA KIT
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Catalog
E043452
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species
Mouse
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GeneGMP-140
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Standard Curve
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Other Species
Human GMP 140 ELISA KitHuman GMP-140 ELISA KitHuman SP Selectin/GMP 140/CD62P ELISA KitMouse SP-Selectin/GMP-140/CD62P ELISA Kit
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SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
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Intended UseMouse GMP-140 ELISA Kit allows for the in vitro quantitative determination of GMP-140 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
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StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
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Product Categories/FamilyCardiovascular
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Product Description
specificalIntended Uses: This GMP-140 ELISA kit is intended Laboratory for research use only and is not for use in diagnostic or therapeutic procedures.The Stop Solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration of GMP-140 in the sample, this GMP-140 ELISA Kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versus GMP-140 concentration. The concentration of GMP-140 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Principle of the Assay: This GMP-140 enzyme linked immunosorbent assay applies a technique called a quantitative sandwich immunoassay. The microtiter plate provided in this kit has been pre-coated with a monoclonal antibody specific for GMP-140. Standards or samples are then added to the microtiter plate wells and GMP-140 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of GMP-140 present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for GMP-140 are added to each well to "sandwich" the GMP-140 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, A and B substrate solution is added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period.Only those wells that contain GMP-140 and enzyme-conjugated antibody will exhibit a change in colour. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the colour change is measured spectrophotometrically at a wavelength of 450 nm.
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