-
Alternative name
Mouse cryptdin-1 ELISA Kit;Mouse defensin-related cryptdin peptide ELISA Kit;Mouse Defcr ELISA Kit;Mouse Defcr1 ELISA Kit;Mouse defensin, alpha 1 ELISA Kit;Mouse alpha-defensin 1 ELISA Kit;Mouse defensin related cryptdin peptide 1 ELISA Kit;Mouse defensin-1 ELISA Kit;
-
Catalog
E043401
-
species
Mouse
-
GeneDefa1
-
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
-
Sensitivity<0.234ng/mL
-
Intended UseMouse Defa1 ELISA Kit allows for the in vitro quantitative determination of Defa1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
-
StorageStore the whole ELISA kit at 4℃
-
Product Description
specificalPrinciple of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. anti-Mouse Defa1 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Mouse Defa1 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Mouse Defa1 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Mouse Defa1 can be calculated.
-
Mouse Alpha-defensin 1 Protein information
-
Uniprot ID
DEFA1_MOUSE
-
Uniprot AC
P11477; Q61448;
-
UniGene
Mm.425522;
-
GeneID
13216
-
KEGG
mmu:13216;
-
Mouse Alpha-defensin 1 Protein SEQUENCE
-
SEQUENCE 93 AA; 10461 MW; 3CD0B6FB9644A22D CRC64;
MKKLVLLFAL VLLGFQVQAD SIQNTDEETK TEEQPGEEDQ AVSVSFGDPE
GTSLQEESLR
DLVCYCRSRG CKGRERMNGT CRKGHLLYTL CCR
-