Mouse a1AT ELISA Kit

Contact Us
  • Alternative name

    Alpha-1-antitrypsin ELISA KIT; Alpha-1 protease inhibitor ELISA KIT; Alpha-1-antiproteinase ELISA KIT; Serpin A1SERPINA1 ELISA KIT; AAT ELISA KIT; PI ELISA KIT; SPAAT ELISA KIT

  • Catalog
    E043327
  • species
    Mouse
  • Genea1AT
  • Standard CurveMouse a1AT ELISA Kit
  • Other Species Human a1AT ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Alpha-1-Antitrypsin (a1AT). No significant cross-reactivity or interference between Alpha-1-Antitrypsin (a1AT) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 13.2ng/mL.
  • Intended UseMouse a1AT ELISA Kit allows for the in vitro quantitative determination of a1AT , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageFor 5-7days:Store the whole kit at 4℃
    For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
  • Product Description
    specifical
    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Alpha-1-Antitrypsin (a1AT). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Alpha-1-Antitrypsin (a1AT). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Alpha-1-Antitrypsin (a1AT), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Alpha-1-Antitrypsin (a1AT) in the samples is then determined by comparing the O.D. of the samples to the standard curve.


Related products with Mouse a1AT ELISA Kit

Inquiry About Mouse a1AT ELISA Kit If you hope to order it or contact us directly, please contact us via sales@biobool.com. We will get back to you in 12hrs,Thanks!