Mouse alpha-SYNo ELISA Kit

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  • Alternative name

    Alpha-synuclein ELISA KIT; Non-A beta component of AD amyloid ELISA KIT; Non-A4 component of amyloid precursor ELISA KIT; NACPSNCA ELISA KIT; NACP ELISA KIT; PARK1 ELISA KIT; NACP ELISA KIT

  • Catalog
  • species
  • Genealpha-SYNo
  • Standard CurveMouse alpha-SYNo ELISA Kit
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Intended UseMouse alpha-SYNo ELISA Kit allows for the in vitro quantitative determination of alpha-SYNo , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageFor 5-7days:Store the whole kit at 4℃
    For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
  • Product Categories/FamilyNeurobiology
  • Product Description
    Principle of the Assay: alpha-SYNo ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific foralpha-SYNo. Standards or samples are then added to the microtiter plate wells and alpha-SYNo if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of alpha-SYNo present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for alpha-SYNo are added to each well to "sandwich" the alpha-SYNo immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain alpha-SYNo and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The alpha-SYNo concentration in each sample is interpolated from this standard curve.

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