SpecificityThis assay has high sensitivity and excellent specificity for detection of mouse AIF1. No significant cross-reactivity or interference between mouse AIF1 and analogues was observed.
Intended UseMouse Aif1 ELISA Kit allows for the in vitro quantitative determination of Aif1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description specificalPrinciple of the Assay||This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for AIF1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any AIF1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for AIF1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of AIF1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Mouse Allograft inflammatory factor 1 Protein information
Uniprot ID
AIF1_MOUSE
Uniprot AC
O70200;
UniGene
Mm.10747;
GeneID
11629
KEGG
mmu:11629;
Mouse Allograft inflammatory factor 1 Protein SEQUENCE