Mouse Aldoa ELISA Kit

Alternative name
Mouse Aldolase 1 ELISA Kit;Mouse muscle-type aldolase ELISA Kit;Mouse Aldo1 ELISA Kit;Mouse Aldo-1 ELISA Kit;Mouse aldolase A, fructose-bisphosphate ELISA Kit;Mouse fructose-bisphosphate aldolase A ELISA Kit;Mouse aldolase 1, A isoform ELISA Kit;
Catalog
E043207
species
Mouse
GeneAldoa
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity0.1 ng/mL.
Intended UseMouse Aldoa ELISA Kit allows for the in vitro quantitative determination of Aldoa , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalFor samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate INTENDED USE This ALDOA ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse ALDOA. This ELISA kit for research use only, not for therapeutic or diagnostic applications! PRINCIPLE OF THE ASSAY ALDOA ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-ALDOA antibody and an ALDOA-HRP conjugate. The assay sample and buffer are incubated together with ALDOA-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ALDOA concentration since ALDOA from samples and ALDOA-HRP conjugate compete for the anti-ALDOA antibody binding site. Since the number of sites is limited, as more sites are occupied by ALDOA from the sample, fewer sites are left to bind ALDOA-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ALDOA concentration in each sample is interpolated from this standard curve.
Mouse Fructose-bisphosphate aldolase A Protein information
Uniprot ID ALDOA_MOUSE
Uniprot AC P05064;
UniGene Mm.275831;
GeneID 11674
KEGG mmu:11674;
Mouse Fructose-bisphosphate aldolase A Protein SEQUENCE
SEQUENCE 364 AA; 39356 MW; 0D067F7E4C63E216 CRC64;
MPHPYPALTP EQKKELSDIA HRIVAPGKGI LAADESTGSI AKRLQSIGTE
NTEENRRFYR QLLLTADDRV NPCIGGVILF HETLYQKADD GRPFPQVIKS
KGGVVGIKVD KGVVPLAGTN GETTTQGLDG LSERCAQYKK DGADFAKWRC
VLKIGEHTPS ALAIMENANV LARYASICQQ NGIVPIVEPE ILPDGDHDLK
RCQYVTEKVL AAVYKALSDH HVYLEGTLLK PNMVTPGHAC TQKFSNEEIA
MATVTALRRT VPPAVTGVTF LSGGQSEEEA SINLNAINKC PLLKPWALTF
SYGRALQASA LKAWGGKKEN LKAAQEEYIK RALANSLACQ GKYTPSGQSG
AAASESLFIS NHAY
UCSC uc009jsu.2; mouse.;

Category

Mouse Aldoa ELISA Kit

Mouse Aldolase 1 ELISA Kit;Mouse muscle-type aldolase ELISA Kit;Mouse Aldo1 ELISA Kit;Mouse Aldo-1 ELISA Kit;Mouse aldolase A, fructose-bisphosphate ELISA Kit;Mouse fructose-bisphosphate aldolase A ELISA Kit;Mouse aldolase 1, A isoform ELISA Kit;